The Borrelia hermsii Factor H Binding Protein FhbA Is Not Required for Infectivity in Mice or for Resistance to Human Complement In Vitro

Lindy M. Fine; Daniel P. Miller; Katherine L. Mallory; Brittney K. Tegels; Christopher G. Earnhart; Richard T. Marconi

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The Borrelia hermsii Factor H Binding Protein FhbA Is Not Required for Infectivity in Mice or for Resistance to Human Complement In Vitro

机译：对于小鼠的感染性或体外对人补体的抗性，不需要芽孢疏螺旋体H结合蛋白FhbA

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The primary causative agent of tick-borne relapsing fever in North America is Borrelia hermsii. It has been hypothesized that B. hermsii evades complement-mediated destruction by binding factor H (FH), a host-derived negative regulator of complement. In vitro, B. hermsii produces a single FH binding protein designated FhbA (FH binding protein A). The properties and ligand binding activity of FhbA suggest that it plays multiple roles in pathogenesis. It binds plasminogen and has been identified as a significant target of a B1b B cell-mediated IgM response in mice. FhbA has also been explored as a potential diagnostic antigen for B. hermsii infection in humans. The ability to test the hypothesis that FhbA is a critical virulence factor in vivo has been hampered by the lack of well-developed systems for the genetic manipulation of the relapsing fever spirochetes. In this report, we have successfully generated a B. hermsii fhbA deletion mutant (the B. hermsii YORΔfhbA strain) through allelic exchange mutagenesis. Deletion of fhbA abolished FH binding by the YORΔfhbA strain and eliminated cleavage of C3b on the cell surface. However, the YORΔfhbA strain remained infectious in mice and retained resistance to killing in vitro by human complement. Collectively, these results indicate that B. hermsii employs an FhbA/FH-independent mechanism of complement evasion that allows for resistance to killing by human complement and persistence in mice.

机译：北美tick传播复发热的主要病原体是黑氏疏螺旋体。据推测，贺氏芽孢杆菌通过结合因子H（FH）逃避补体介导的破坏，结合因子H是宿主衍生的补体负调节剂。在体外，疱疹双歧杆菌产生一种称为FhbA的FH结合蛋白（FH结合蛋白A）。 FhbA的性质和配体结合活性表明，它在发病机理中起着多种作用。它结合纤溶酶原，已被确定为小鼠中B1b B细胞介导的IgM反应的重要靶标。 FhbA也已被开发为人类感染埃希氏杆菌的潜在诊断抗原。由于缺乏对复发性发热螺旋体进行基因操作的完善系统，因此无法检验FhbA是体内关键毒力因子这一假说的能力。在本报告中，我们已经通过等位基因交换诱变成功地产生了一个赫氏芽孢杆菌 fhbA 缺失突变体（赫氏芽孢杆菌YORΔ fhbA 菌株）。删除 fhbA 消除了YORΔ fhbA 菌株的FH结合，消除了C3b在细胞表面的裂解。然而，YORΔ fhbA 株在小鼠中仍具有传染性，并保留了对人补体杀死体外的抗性。总体而言，这些结果表明，赫氏芽孢杆菌采用了FhbA / FH独立的补体逃逸机制，该机制可抵抗人类补体的杀伤和在小鼠中的持久性。 展开▼

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《Infection and immunity》 |2014年第8期|共9页

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Lindy M. Fine; Daniel P. Miller; Katherine L. Mallory; Brittney K. Tegels; Christopher G. Earnhart; Richard T. Marconi;
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中图分类医学免疫学;

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1. Identification of an Antiparallel Coiled-Coil/Loop Domain Required for Ligand Binding by the Borrelia hermsii FhbA Protein: Additional Evidence for the Role of FhbA in the Host-Pathogen Interaction [J] . Kelley M. Hovis, John C. Freedman, Hongming Zhang, Infection and immunity . 2008,第5期

机译：鉴定由伯氏疏螺旋体FhbA蛋白进行配体结合所需的反平行螺旋线圈/环状结构域：FhbA在宿主-病原体相互作用中的作用的其他证据

2. Molecular Analyses of the Interaction of Borrelia hermsii FhbA with the Complement Regulatory Proteins Factor H and Factor H-Like Protein 1 [J] . Kelley M. Hovis, Janice P. Jones, Tania Sadlon, Infection and immunity . 2006,第4期

机译：鲍氏疏螺旋体FhbA与补体调节蛋白因子H和因子H样蛋白1相互作用的分子分析

3. Immunological and Molecular Analyses of the Borrelia hermsii Factor H and Factor H-Like Protein 1 Binding Protein, FhbA: Demonstration of Its Utility as a Diagnostic Marker and Epidemiological Tool for Tick-Borne Relapsing Fever [J] . Kelley M. Hovis, Martin E. Schriefer, Sonia Bahlani, Infection and immunity . 2006,第8期

机译：伯氏疏螺旋体H因子和H因子样蛋白1结合蛋白FhbA的免疫学和分子分析：证明其作为Tick-Borne复发热的诊断标志物和流行病学工具的效用

4. Humanized Recombinant Vaccinia Virus Complement Control Protein (hrVCP) with Three Amino Acid Changes, H98Y, E102K, and E120K Creating an Additional Putative Heparin Binding Site, Is 100-fold More Active Than rVCP in Blocking Both Classical and Alternative Complement Pathways [C] . YOHANNES T. GHEBREMARIAM, ODUTAYO O. ODUNUGA, KRISTEN JANSE, International Conference on Natural Products and Molecular Medicine . 2005

机译：人源化重组疫苗病毒补蛋白（HRVCP）具有三个氨基酸变化，H98Y，E102K和E120K产生另外的肝素结合位点，比RVCP在阻断经典和替代的补体途径时，100倍。

5. The identification and characterization of a fibronectin-binding protein of Borrelia hermsii expressed in the blood during experimental relapsing fever. [D] . Lewis, Eric R. G. 2013

机译：实验性复发性发热期间血液中表达的疏螺旋体纤连蛋白结合蛋白的鉴定和表征。

6. The Borrelia hermsii Factor H Binding Protein FhbA Is Not Required for Infectivity in Mice or for Resistance to Human Complement In Vitro [O] . Lindy M. Fine, Daniel P. Miller, Katherine L. Mallory, 2014

机译：对于小鼠的感染性或体外对人补体的抗性不需要博氏疏螺旋体H结合蛋白FhbA

7. The Borrelia hermsii Factor H Binding Protein FhbA Is Not Required for Infectivity in Mice or for Resistance to Human ComplementIn Vitro [O] . Lindy M. Fine, Daniel P. Miller, Katherine L. Mallory, 2014

机译：Borrelia Hermsii因子H结合蛋白FHBA不需要小鼠的感染性或抗人类互补性体外

1. 小鼠遗传背景对赫姆斯疏螺旋体感染的影响 [J] . 陆巍 ,陶玉芬 ,程美慧 . 中国动物传染病学报 . 2019,第006期

2. 大枣多糖体外抗补体活性及促进小鼠脾细胞的增殖作用 [J] . 张庆 ,林勤保 ,等 . 中药药理与临床 . 1998,第005期

3. 用单克隆抗体及补体体外清除T细胞的小鼠异基因骨髓移植 [J] . 杨骏 ,谢蜀生 ,龙振洲 . 中华器官移植杂志 . 1991,第001期

4. 赖型钩端螺旋体内鞭毛蛋白基因的体外扩增、分子克隆、核苷酸序列分析及其编码33kDa内鞭毛蛋白对BALB/c小鼠的兔疫/保护作用 [J] . 戴保民 ,陈庄 ,阎和平 . 医学研究杂志 . 1998,第006期

5. 脂肪酸结合蛋白抑制剂在小鼠体外对胰岛β细胞遭受巨噬细胞介导的炎症损伤的保护作用 [J] . 刘石平 ,贺利宁 ,肖扬 . 中华医学杂志 . 2012,第046期

6. 人工与自然血吸虫感染性钉螺对小鼠的感染性研究 [C] . 喻鑫玲 ,周杰 ,胡君健 . 第11届中南地区实验动物科技交流会 . 2011

7. 梅毒螺旋体体外活化人脑微血管内皮细胞的实验研究 [A] . 吴凡 . 2017

1. 小鼠巨噬细胞人补体膜攻击复合物体外模型及构建和应用 [P] . 中国专利： CN110241082A . 2019-09-17

2. 使用不需要钙黏着蛋白受体的毒素遏制昆虫对苏云金芽孢杆菌CRY毒素的抗性 [P] . 中国专利： CN104745603B . 2018.08.21

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机译：基因工程丙型肝炎病毒（hcv）核酸克隆，质粒克隆，其中包含从全长hcv cdna转录而来的真人大小的hcv cdna，dna或rna，并通过非人类动物的hcv转染和体外感染宿主细胞系被hcv感染，hcv病毒颗粒，体外测试系统，无hcv细胞，hcv疫苗和hcv测试仪的感染以及鉴定允许的细胞系和允许感染hvv的动物的过程，以选择具有以下适应性突变的hcv：允许在允许的细胞系中进行更高水平的hvv复制，以hvv感染动物，在体外传播hvv，用异源基因转导易感染hvv的动物，产生hvv病毒颗粒，hvv颗粒蛋白和hvv抗体分离能够调节hcv复制的试剂，制备hcv核酸并检测患者生物样品中的hcv抗体。

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机译：检测和/或滴定非常规可传播试剂（ATNC）或蛋白质构象病理的体外方法; mpetodos体外评估和/或控制过程或程序;评估化合物PA调节生物产物感染性的能力的体外方法;海绵状脑病的体外诊断方法可在个体或非人类动物中传播。

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机译：测定从人恶性肿瘤和由LDH病毒感染白细胞介导的C3H / He2k感染小鼠血清中获得的免疫功能性抗原性核糖核酸（RNA）的体外反应方法

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The Borrelia hermsii Factor H Binding Protein FhbA Is Not Required for Infectivity in Mice or for Resistance to Human Complement In Vitro

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