...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Infection and immunity >Aggregatibacter actinomycetemcomitans Outer Membrane Vesicles Are Internalized in Human Host Cells and Trigger NOD1- and NOD2-Dependent NF-κB Activation
【24h】

Aggregatibacter actinomycetemcomitans Outer Membrane Vesicles Are Internalized in Human Host Cells and Trigger NOD1- and NOD2-Dependent NF-κB Activation

机译：聚合菌放线菌外膜囊泡在人类宿主细胞中内化并触发依赖NOD1和NOD2的NF-κB激活

获取原文
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Aggregatibacter actinomycetemcomitans is an oral and systemic pathogen associated with aggressive forms of periodontitis and with endocarditis. We recently demonstrated that outer membrane vesicles (OMVs) disseminated by A. actinomycetemcomitans could deliver multiple proteins, including biologically active cytolethal distending toxin (CDT), into the cytosol of HeLa cells and human gingival fibroblasts (HGF). In the present work, we have used immunoelectron and confocal microscopy analysis and fluorescently labeled vesicles to further investigate mechanisms for A. actinomycetemcomitans OMV-mediated delivery of bacterial antigens to these host cells. Our results supported that OMVs were internalized into the perinuclear region of HeLa cells and HGF. Colocalization analysis revealed that internalized OMVs colocalized with the endoplasmic reticulum and carried antigens, detected using an antibody specific to whole A. actinomycetemcomitans serotype a cells. Consistent with OMV internalization mediating intracellular antigen exposure, the vesicles acted as strong inducers of cytoplasmic peptidoglycan sensor NOD1- and NOD2-dependent NF-κB activation in human embryonic kidney cells. Moreover, NOD1 was the main sensor of OMV-delivered peptidoglycan in myeloid THP1 cells, contributing to the overall inflammatory responses induced by the vesicles. This work reveals a role of A. actinomycetemcomitans OMVs as a trigger of innate immunity via carriage of NOD1- and NOD2-active pathogen-associated molecular patterns (PAMPs).
机译：放线杆菌聚合酶是口腔和全身性病原体，与侵袭性牙周炎和心内膜炎有关。我们最近证明，由放线放线杆菌（A. actinomycetemcomitans）传播的外膜囊泡（OMV）可以将多种蛋白质（包括具有生物活性的致死性膨大毒素（CDT））传递到HeLa细胞和人牙龈成纤维细胞（HGF）的细胞质中。在目前的工作中，我们使用了免疫电子和共聚焦显微镜分析以及荧光标记的囊泡，以进一步研究A.放线杆菌介导的OMV介导的细菌抗原传递到这些宿主细胞的机制。我们的结果支持OMV被内化到HeLa细胞和HGF的核周区域中。共定位分析显示，内化的OMV与内质网共定位并携带抗原，这是使用对整个A.放线杆菌血清A型细胞特异的抗体检测到的。与介导细胞内抗原暴露的OMV内部化一致，囊泡在人类胚胎肾细胞中充当细胞质肽聚糖传感器NOD1和NOD2依赖性NF-κB激活的强诱导剂。此外，NOD1是髓样THP1细胞中OMV传递的肽聚糖的主要传感器，有助于囊泡诱导的整体炎症反应。这项工作揭示了通过携带NOD1和NOD2活性病原体相关分子模式（PAMP）携带放线放线杆菌OMVs作为先天免疫触发的作用。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号