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首页> 外文期刊>Infection and immunity >Two-Photon Intravital Imaging of Lungs during Anthrax Infection Reveals Long-Lasting Macrophage-Dendritic Cell Contacts
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Two-Photon Intravital Imaging of Lungs during Anthrax Infection Reveals Long-Lasting Macrophage-Dendritic Cell Contacts

机译：炭疽感染期间肺部的双光子活体成像显示持久的巨噬细胞-树突状细胞接触。

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The dynamics of the lung immune system at the microscopic level are largely unknown because of inefficient methods of restraining chest motion during image acquisition. In this study, we developed an improved intravital method for two-photon lung imaging uniquely based on a posteriori parenchymal tissue motion correction. We took advantage of the alveolar collagen pattern given by the second harmonic generation signal as a reference for frame registration. We describe here for the first time a detailed dynamic account of two major lung immune cell populations, alveolar macrophages and CD11b-positive dendritic cells, during homeostasis and infection by spores of Bacillus anthracis, the agent of anthrax. We show that after alveolar macrophages capture spores, CD11b-positive dendritic cells come in prolonged contact with infected macrophages. Dendritic cells are known to carry spores to the draining lymph nodes and elicit the immune response in pulmonary anthrax. The intimate and long-lasting contacts between these two lines of defense may therefore coordinate immune responses in the lung through an immunological synapse-like process.
机译：由于在图像采集过程中无法有效地抑制胸部运动，因此在微观层面上肺免疫系统的动力学尚不清楚。在这项研究中，我们基于后壁实质组织运动校正独特地开发了一种改进的用于双光子肺成像的活体检查方法。我们利用二次谐波生成信号给出的肺泡胶原蛋白模式作为帧配准的参考。我们在这里首次描述了动态平衡和炭疽芽孢杆菌（炭疽病的病原体）的孢子感染过程中两个主要的肺免疫细胞群，肺泡巨噬细胞和CD11b阳性树突状细胞的详细动态描述。我们显示，肺泡巨噬细胞捕获孢子后，CD11b阳性树突状细胞与感染的巨噬细胞长时间接触。已知树突状细胞将孢子携带至引流的淋巴结并引发肺炭疽的免疫反应。因此，这两道防线之间的紧密而持久的接触可以通过免疫突触样过程协调肺中的免疫反应。

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