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首页> 外文期刊>Infection and immunity >The Agr Quorum-Sensing System Regulates Fibronectin Binding but Not Hemolysis in the Absence of a Functional Electron Transport Chain
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The Agr Quorum-Sensing System Regulates Fibronectin Binding but Not Hemolysis in the Absence of a Functional Electron Transport Chain

机译：在没有功能性电子传输链的情况下，农业群仲裁感测系统调节纤连蛋白结合但不溶血

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Staphylococcus aureus is responsible for numerous chronic and recurrent infections, which are frequently associated with the emergence of small-colony variants (SCVs) that lack a functional electron transport chain. SCVs exhibit enhanced expression of fibronectin-binding protein (FnBP) and greatly reduced hemolysin production, although the basis for this is unclear. One hypothesis is that these phenotypes are a consequence of the reduced Agr activity of SCVs, while an alternative is that the lack of a functional electron transport chain and the resulting reduction in ATP production are responsible. Disruption of the electron transport chain of S. aureus genetically (hemB and menD) or chemically, using 2-n-heptyl-4-hydroxyquinoline N-oxide (HQNO), inhibited both growth and Agr activity and conferred an SCV phenotype. Supplementation of the culture medium with synthetic autoinducing peptide (sAIP) significantly increased Agr expression in both hemB mutant strains and S. aureus grown with HQNO and significantly reduced staphylococcal adhesion to fibronectin. However, sAIP did not promote hemolysin expression in hemB mutant strains or S. aureus grown with HQNO. Therefore, while Agr regulates fibronectin binding in SCVs, it cannot promote hemolysin production in the absence of a functional electron transport chain.
机译：金黄色葡萄球菌是造成许多慢性和复发性感染的原因，这些感染通常与缺乏功能性电子传输链的小菌落变种（SCV）的出现有关。 SCV表现出增强的纤连蛋白结合蛋白（FnBP）的表达，并大大减少了溶血素的产生，尽管其基础尚不清楚。一种假设是这些表型是SCV的Agr活性降低的结果，而另一种选择是缺乏功能性电子传输链和由此导致的ATP产量降低。使用2- n -庚基-4-羟基喹啉通过遗传或化学方式破坏金黄色葡萄球菌的电子传输链（ hemB 和 menD ） N -氧化物（HQNO）抑制生长和Agr活性，并赋予SCV表型。在培养基中添加合成的自诱导肽（sAIP）可以显着增加在 hemB 突变株和HQNO培养的金黄色葡萄球菌中Agr的表达，并显着降低葡萄球菌对纤连蛋白的粘附。然而，sAIP不能促进HQNO培养的 hemB 突变株或金黄色葡萄球菌的溶血素表达。因此，尽管Agr调节SCV中的纤连蛋白结合，但在没有功能性电子传输链的情况下，它不能促进溶血素的产生。

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