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首页> 外文期刊>Infection and immunity >Escherichia coli Uropathogenesis In Vitro: Invasion, Cellular Escape, and Secondary Infection Analyzed in a Human Bladder Cell Infection Model
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Escherichia coli Uropathogenesis In Vitro: Invasion, Cellular Escape, and Secondary Infection Analyzed in a Human Bladder Cell Infection Model

机译：大肠杆菌尿毒症的体外发病机制：入侵，细胞逃逸和继发性感染在人类膀胱细胞感染模型中进行了分析。

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Uropathogenic Escherichia coli (UPEC) strains are capable of invading bladder epithelial cells (BECs) on the bladder luminal surface. Based primarily on studies in mouse models, invasion is proposed to trigger an intracellular uropathogenic cascade involving intracellular bacterial proliferation followed by escape of elongated, filamentous bacteria from colonized BECs. UPEC filaments on the mouse bladder epithelium are able to revert to rod-shaped bacteria, which are believed to invade neighboring cells to initiate new rounds of intracellular colonization. So far, however, these late-stage infection events have not been replicated in vitro. We have established an in vitro model of human bladder cell infection by the use of a flow chamber (FC)-based culture system, which allows investigation of steps subsequent to initial invasion. Short-term bacterial colonization on the FC-BEC layer led to intracellular colonization. Exposing invaded BECs to a flow of urine, i.e., establishing conditions similar to those faced by UPEC reemerging on the bladder luminal surface, led to outgrowth of filamentous bacteria similar to what has been reported to occur in mice. These filaments were capable of reverting to rods that could invade other BECs. Hence, under growth conditions established to resemble those present in vivo, the elements of the proposed uropathogenic cascade were inducible in a human BEC model system. Here, we describe the model and show how these characteristics are reproduced in vitro.
机译：致病性大肠杆菌（UPEC）菌株能够侵袭膀胱腔表面的膀胱上皮细胞（BEC）。主要基于小鼠模型的研究，提出了入侵以触发细胞内尿毒症级联反应，该过程涉及细胞内细菌增殖，然后从定殖的BEC中逃逸出细长的丝状细菌。小鼠膀胱上皮细胞上的UPEC细丝能够还原为杆状细菌，据信该细菌会入侵邻近细胞以启动新一轮细胞内定植。但是，到目前为止，这些晚期感染事件尚未在体外复制。我们通过使用基于流室（FC）的培养系统建立了人类膀胱细胞感染的体外模型，该模型可以研究初始侵袭后的步骤。 FC-BEC层上的短期细菌定植导致细胞内定植。将侵入的BEC暴露于尿流中，即建立与UPEC在膀胱腔表面重新出现的条件相似的条件，导致丝状细菌的生长与已报道的小鼠相似。这些细丝能够还原成可能侵入其他BEC的棒。因此，在确定的生长条件类似于体内存在的生长条件下，拟议的尿路致病级联反应的元件可在人BEC模型系统中诱导。在这里，我们描述模型并显示如何在体外复制这些特征。

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