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首页> 外文期刊>Infection and immunity >The Streptomycin-Treated Mouse Intestine Selects Escherichia coli envZ Missense Mutants That Interact with Dense and Diverse Intestinal Microbiota
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The Streptomycin-Treated Mouse Intestine Selects Escherichia coli envZ Missense Mutants That Interact with Dense and Diverse Intestinal Microbiota

机译：链霉素处理的小鼠肠道选择与致密和多种肠道菌群相互作用的大肠杆菌envZ错义突变体。

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Previously, we reported that the streptomycin-treated mouse intestine selected nonmotile Escherichia coli MG1655 flhDC deletion mutants of E. coli MG1655 with improved colonizing ability that grow 15% faster in vitro in mouse cecal mucus and 15 to 30% faster on sugars present in mucus (M. P. Leatham et al., Infect. Immun. 73:8039–8049, 2005). Here, we report that the 10 to 20% remaining motile E. coli MG1655 are envZ missense mutants that are also better colonizers of the mouse intestine than E. coli MG1655. One of the flhDC mutants, E. coli MG1655 ΔflhD, and one of the envZ missense mutants, E. coli MG1655 mot-1, were studied further. E. coli MG1655 mot-1 is more resistant to bile salts and colicin V than E. coli MG1655 ΔflhD and grows ca. 15% slower in vitro in mouse cecal mucus and on several sugars present in mucus compared to E. coli MG1655 ΔflhD but grows 30% faster on galactose. Moreover, E. coli MG1655 mot-1 and E. coli MG1655 ΔflhD appear to colonize equally well in one intestinal niche, but E. coli MG1655 mot-1 appears to use galactose to colonize a second, smaller intestinal niche either not colonized or colonized poorly by E. coli MG1655 ΔflhD. Evidence is also presented that E. coli MG1655 is a minority member of mixed bacterial biofilms in the mucus layer of the streptomycin-treated mouse intestine. We offer a hypothesis, which we call the “Restaurant” hypothesis, that explains how nutrient acquisition in different biofilms comprised of different anaerobes can account for our results.
机译：以前，我们报道了链霉素处理的小鼠肠道选择了大肠杆菌MG1655的非运动型大肠杆菌MG1655 flhDC 缺失突变体，其克隆能力提高了，在体外体外生长了15％。小鼠盲肠粘液，对粘液中存在的糖类的吸收速度要快15％至30％（MP Leatham等人，Infect。Immun。73：8039–8049，2005）。在这里，我们报告说，剩余的10％到20％的运动型大肠杆菌MG1655是 envZ 错义突变体，与大肠杆菌MG1655相比，它们也是小鼠肠道的更好定居者。 flhDC 突变体之一，大肠杆菌MG1655Δ flhD ，以及 envZ 错义突变体之一，大肠杆菌MG1655 mot-1，被进一步研究。与大肠杆菌MG1655Δ flhD 相比，大肠杆菌MG1655 mot-1对胆盐和大肠菌素V的耐药性更高。与大肠杆菌MG1655Δ flhD 相比，小鼠盲肠粘液和粘液中存在的几种糖的体外慢15％，但半乳糖生长快30％。此外，E。coli MG1655 mot-1和E. coli MG1655Δ flhD 在一个小生境中似乎同样定殖，而E. coli MG1655 mot-1似乎在半乳糖中定居。大肠杆菌MG1655Δ flhD 未定殖或定殖的肠道小生境。也有证据表明，大肠杆菌MG1655是链霉素处理过的小鼠肠道粘液层中混合细菌生物膜的少数成员。我们提供了一个假设，我们称之为“餐馆”假设，该假设解释了由不同厌氧菌组成的不同生物膜中营养的获取如何解释我们的结果。

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