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首页> 外文期刊>Infection and immunity >Global Transcriptome Analysis of Staphylococcus aureus Biofilms in Response to Innate Immune Cells
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Global Transcriptome Analysis of Staphylococcus aureus Biofilms in Response to Innate Immune Cells

机译：全球转录组分析金黄色葡萄球菌生物膜对先天免疫细胞的反应。

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The potent phagocytic and microbicidal activities of neutrophils and macrophages are among the first lines of defense against bacterial infections. Yet Staphylococcus aureus is often resistant to innate immune defense mechanisms, especially when organized as a biofilm. To investigate how S. aureus biofilms respond to macrophages and neutrophils, gene expression patterns were profiled using Affymetrix microarrays. The addition of macrophages to S. aureus static biofilms led to a global suppression of the biofilm transcriptome with a wide variety of genes downregulated. Notably, genes involved in metabolism, cell wall synthesis/structure, and transcription/translation/replication were among the most highly downregulated, which was most dramatic at 1 h compared to 24 h following macrophage addition to biofilms. Unexpectedly, few genes were enhanced in biofilms after macrophage challenge. Unlike coculture with macrophages, coculture of S. aureus static biofilms with neutrophils did not greatly influence the biofilm transcriptome. Collectively, these experiments demonstrate that S. aureus biofilms differentially modify their gene expression patterns depending on the leukocyte subset encountered.
机译：中性粒细胞和巨噬细胞的强吞噬和杀微生物活性是抵抗细菌感染的第一道防线。然而，金黄色葡萄球菌通常对先天的免疫防御机制有抵抗力，尤其是当组织成生物膜时。为了研究金黄色葡萄球菌生物膜对巨噬细胞和嗜中性粒细胞的反应，使用Affymetrix微阵列分析了基因表达模式。向金黄色葡萄球菌静态生物膜中添加巨噬细胞导致生物膜转录组的整体抑制，其中多种基因被下调。值得注意的是，参与代谢，细胞壁合成/结构和转录/翻译/复制的基因被高度下调，与巨噬细胞加入生物膜后24小时相比，在1小时内最显着。出乎意料的是，巨噬细胞攻击后，生物膜中几乎没有基因被增强。与巨噬细胞共培养不同，金黄色葡萄球菌静态生物膜与中性粒细胞的共培养对生物膜转录组没有太大影响。总的来说，这些实验表明，金黄色葡萄球菌生物膜根据遇到的白细胞亚群差异地修饰其基因表达模式。

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