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首页> 外文期刊>Infection and immunity >Adhesive Properties of YapV and Paralogous Autotransporter Proteins of Yersinia pestis
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Adhesive Properties of YapV and Paralogous Autotransporter Proteins of Yersinia pestis

机译：YapV和鼠疫耶尔森氏菌的旁源自转运蛋白的粘附特性。

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Yersinia pestis is the causative agent of plague. This bacterium evolved from an ancestral enteroinvasive Yersinia pseudotuberculosis strain by gene loss and acquisition of new genes, allowing it to use fleas as transmission vectors. Infection frequently leads to a rapidly lethal outcome in humans, a variety of rodents, and cats. This study focuses on the Y. pestis KIM yapV gene and its product, recognized as an autotransporter protein by its typical sequence, outer membrane localization, and amino-terminal surface exposure. Comparison of Yersinia genomes revealed that DNA encoding YapV or each of three individual paralogous proteins (YapK, YapJ, and YapX) was present as a gene or pseudogene in a strain-specific manner and only in Y. pestis and Y. pseudotuberculosis. YapV acted as an adhesin for alveolar epithelial cells and specific extracellular matrix (ECM) proteins, as shown with recombinant Escherichia coli, Y. pestis, or purified passenger domains. Like YapV, YapK and YapJ demonstrated adhesive properties, suggesting that their previously related in vivo activity is due to their capacity to modulate binding properties of Y. pestis in its hosts, in conjunction with other adhesins. A differential host-specific type of binding to ECM proteins by YapV, YapK, and YapJ suggested that these proteins participate in broadening the host range of Y. pestis. A phylogenic tree including 36 Y. pestis strains highlighted an association between the gene profile for the four paralogous proteins and the geographic location of the corresponding isolated strains, suggesting an evolutionary adaption of Y. pestis to specific local animal hosts or reservoirs.
机译：鼠疫耶尔森氏菌是鼠疫的病原体。该细菌通过基因损失和获取新基因从祖先肠侵袭性耶尔森氏菌假结核菌演变而来，使其可以将跳蚤用作传播载体。感染经常导致人类，各种啮齿动物和猫类迅速死亡。这项研究的重点是鼠疫耶尔森氏菌KIM yapV基因及其产物，通过其典型序列，外膜定位和氨基末端表面暴露被识别为自转运蛋白。耶尔森氏菌基因组的比较表明，编码YapV或三种单独的旁系蛋白（YapK，YapJ和YapX）中的每一种的DNA以菌株特异性方式作为基因或假基因存在，并且仅在鼠疫耶尔森氏菌和假结核耶尔森氏菌中存在。 YapV充当肺泡上皮细胞和特定细胞外基质（ECM）蛋白的粘附素，如重组大肠杆菌，鼠疫耶尔森氏菌或纯化的过客域所示。像YapV一样，YapK和YapJ也表现出粘附特性，这表明它们先前相关的体内活性是由于其与其他粘附素一起调节其鼠疫耶尔森氏菌的结合特性的能力。 YapV，YapK和YapJ与ECM蛋白结合的宿主特异性差异类型表明，这些蛋白质参与了鼠疫耶尔森氏菌宿主范围的扩大。包括36个鼠疫耶尔森氏菌菌株的系统树突出显示了四种旁源蛋白质的基因谱与相应分离菌株的地理位置之间的关联，表明鼠疫耶尔森氏菌对特定的局部动物宿主或贮藏库的进化适应性。

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