• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Infection and immunity >Characterization of Clostridium perfringens TpeL Toxin Gene Carriage, Production, Cytotoxic Contributions, and Trypsin Sensitivity
【24h】

Characterization of Clostridium perfringens TpeL Toxin Gene Carriage, Production, Cytotoxic Contributions, and Trypsin Sensitivity

机译：产气荚膜梭菌TpeL毒素基因运输，产生，细胞毒性贡献和胰蛋白酶敏感性的表征。

获取原文
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Large clostridial toxins (LCTs) are produced by at least four pathogenic clostridial species, and several LCTs are proven pivotal virulence factors for both human and veterinary diseases. TpeL is a recently identified LCT produced by Clostridium perfringens that has received relatively limited study. In response, the current study surveyed carriage of the tpeL gene among different C. perfringens strains, detecting this toxin gene in some type A, B, and C strains but not in any type D or E strains. This study also determined that all tested strains maximally produce, and extracellularly release, TpeL at the late-log or early-stationary growth stage during in vitro culture, which is different from the maximal late-stationary-phase production reported previously for other LCTs and for TpeL production by C. perfringens strain JIR12688. In addition, the present study found that TpeL levels in culture supernatants can be repressed by either glucose or sucrose. It was also shown that, at natural production levels, TpeL is a significant contributor to the cytotoxic activity of supernatants from cultures of tpeL-positive strain CN3685. Lastly, this study identified TpeL, which presumably is produced in the intestines during diseases caused by TpeL-positive type B and C strains, as a toxin whose cytotoxicity decreases after treatment with trypsin; this finding may have pathophysiologic relevance by suggesting that, like beta toxin, TpeL contributes to type B and C infections in hosts with decreased trypsin levels due to disease, diet, or age.
机译：大的梭菌毒素（LCT）至少由四种致病梭菌产生，并且几种LCT已被证明是人类和兽医疾病的关键毒力因子。 TpeL是产气荚膜梭状芽孢杆菌最近鉴定出的LCT，其研究相对有限。作为回应，当前的研究调查了 tpeL 基因在不同产气荚膜梭菌菌株之间的携带情况，在某些A，B和C型菌株中检测到了该毒素基因，但在任何D型或E型菌株中均未检测到。这项研究还确定了所有受试菌株在体外培养过程中的对数晚期或静止早期生长阶段都最大程度地产生TpeL，并在细胞外释放，这不同于最大的静止后期阶段。先前报道的产气荚膜梭菌菌株JIR12688用于其他LCT和TpeL的产量。此外，本研究发现培养上清液中的TpeL水平可以被葡萄糖或蔗糖抑制。还显示出，在自然生产水平下，TpeL是 tpeL 阳性菌株CN3685培养物上清液细胞毒活性的重要贡献者。最后，这项研究确定了TpeL，它可能是由TpeL阳性B型和C型菌株引起的疾病在肠道中产生的一种毒素，经胰蛋白酶处理后其细胞毒性降低。这项发现可能与病理生理有关，暗示像β毒素一样，TpeL会导致由于疾病，饮食或年龄而导致胰蛋白酶水平降低的宿主的B型和C型感染。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号