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首页> 外文期刊>Infection and immunity >The Staphylococcus aureus Protein-Coding Gene gdpS Modulates sarS Expression via mRNA-mRNA Interaction
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The Staphylococcus aureus Protein-Coding Gene gdpS Modulates sarS Expression via mRNA-mRNA Interaction

机译：金黄色葡萄球菌蛋白编码基因gdpS通过mRNA-mRNA相互作用调节sarS表达

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Staphylococcus aureus is an important Gram-positive pathogen responsible for numerous diseases ranging from localized skin infections to life-threatening systemic infections. The virulence of S. aureus is essentially determined by a wide spectrum of factors, including cell wall-associated proteins and secreted toxins that are precisely controlled in response to environmental changes. GGDEF domain protein from Staphylococcus (GdpS) is the only conserved staphylococcal GGDEF domain protein that is involved not in c-di-GMP synthesis but in the virulence regulation of S. aureus NCTC8325. Our previous study showed that the inactivation of gdpS generates an extensive change of virulence factors together with, in particular, a major Spa (protein A) surface protein. As reported, sarS is a direct positive regulator of spa. The decreased transcript levels of sarS in the gdpS mutant compared with the parental NCTC8325 strain suggest that gdpS affects spa through interaction with sarS. In this study, site mutation and complementary experiments showed that the translation product of gdpS was not involved in the regulation of transcript levels of sarS. We found that gdpS functioned through direct RNA-RNA base pairing with the 5′ untranslated region (5′UTR) of sarS mRNA and that a putative 18-nucleotide region played a significant role in the regulatory process. Furthermore, the mRNA half-life analysis of sarS in the gdpS mutant showed that gdpS positively regulates the mRNA levels of sarS by contributing to the stabilization of sarS mRNA, suggesting that gdpS mRNA may regulate spa expression in an RNA-dependent pathway.
机译：金黄色葡萄球菌是一种重要的革兰氏阳性病原体，可引起多种疾病，从局部皮肤感染到危及生命的全身感染。金黄色葡萄球菌的毒力基本上由多种因素决定，包括细胞壁相关蛋白和分泌毒素，这些毒素可根据环境变化进行精确控制。来自葡萄球菌的GGDEF结构域蛋白（GdpS）是唯一保守的葡萄球菌GGDEF结构域蛋白，其不参与c-di-GMP合成，但参与金黄色葡萄球菌NCTC8325的毒力调节。我们以前的研究表明， gdpS 的失活会引起毒力因子的广泛变化，尤其是主要的Spa（蛋白A）表面蛋白。据报道， sarS 是 spa 的直接正调控因子。与亲本NCTC8325菌株相比， gdpS 突变体中 sarS 的转录水平降低表明， gdpS 通过以下方式影响 spa 与 sarS 进行交互。在这项研究中，位点突变和互补实验表明， gdpS 的翻译产物不参与 sarS 转录水平的调节。我们发现 gdpS 通过与 sarS mRNA的5'非翻译区（5'UTR）的直接RNA-RNA碱基配对起作用，并且假定的18个核苷酸区域发挥了作用。在监管过程中发挥重要作用。此外， gdpS 突变体中 sarS 的mRNA半衰期分析表明， gdpS 积极调节 sarS的mRNA水平 em>通过有助于稳定 sarS mRNA的表达，提示 gdpS mRNA可能通过RNA依赖性途径调控 spa 表达。

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