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首页> 外文期刊>Infection and immunity >Identification and Characterization of Outer Membrane Vesicle-Associated Proteins in Salmonella enterica Serovar Typhimurium
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Identification and Characterization of Outer Membrane Vesicle-Associated Proteins in Salmonella enterica Serovar Typhimurium

机译：沙门氏菌鼠伤寒沙门氏菌中外膜囊泡相关蛋白的鉴定与表征

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Salmonella enterica serovar Typhimurium is a primary cause of enteric diseases and has acquired a variety of virulence factors during its evolution into a pathogen. Secreted virulence factors interact with commensal flora and host cells and enable Salmonella to survive and thrive in hostile environments. Outer membrane vesicles (OMVs) released from many Gram-negative bacteria function as a mechanism for the secretion of complex mixtures, including virulence factors. We performed a proteomic analysis of OMVs that were isolated under standard laboratory and acidic minimal medium conditions and identified 14 OMV-associated proteins that were observed in the OMV fraction isolated only under the acidic minimal medium conditions, which reproduced the nutrient-deficient intracellular milieu. The inferred roles of these 14 proteins were diverse, including transporter, enzyme, and transcriptional regulator. The absence of these proteins influenced Salmonella survival inside murine macrophages. Eleven of these proteins were predicted to possess secretion signal sequences at their N termini, and three (HupA, GlnH, and PhoN) of the proteins were found to be translocated into the cytoplasm of host cells. The comparative proteomic profiling of OMVs performed in this study revealed different protein compositions in the OMVs isolated under the two different conditions, which indicates that the OMV cargo depends on the growth conditions and provides a deeper insight into how Salmonella utilizes OMVs to adapt to environmental changes.
机译：肠炎沙门氏菌鼠伤寒沙门氏菌是肠道疾病的主要原因，在其演变成病原体的过程中获得了多种毒力因子。分泌的毒力因子与共生菌群和宿主细胞相互作用，使沙门氏菌能够在不利的环境中生存和繁衍。从许多革兰氏阴性细菌中释放的外膜囊泡（OMV）是分泌包括毒力因子在内的复杂混合物的一种机制。我们对在标准实验室和酸性最低培养基条件下分离的OMV进行了蛋白质组学分析，并鉴定了仅在酸性最低培养基条件下分离的OMV馏分中观察到的14种OMV相关蛋白，从而再现了营养缺乏的细胞内环境。这14种蛋白质的推测作用是多种多样的，包括转运蛋白，酶和转录调节子。这些蛋白质的缺乏影响了鼠巨噬细胞内沙门氏菌的存活。这些蛋白质中有11个被预测在其N末端具有分泌信号序列，并且其中3个（HupA，GlnH和PhoN）被发现易位到宿主细胞的细胞质中。在这项研究中进行的OMV的蛋白质组比较分析显示，在两种不同条件下分离的OMV中蛋白质组成不同，这表明OMV货物取决于生长条件，并提供了更深入的了解沙门氏菌如何利用OMV适应环境变化的信息。

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