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首页> 外文期刊>International Journal of Molecular Sciences >Nuclear and Cytoplasmic Soluble Proteins Extraction from a Small Quantity of Drosophila’s Whole Larvae and Tissues
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Nuclear and Cytoplasmic Soluble Proteins Extraction from a Small Quantity of Drosophila’s Whole Larvae and Tissues

机译:少量果蝇整个幼虫和组织中的核和细胞质可溶性蛋白的提取

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The identification and study of protein’s function in several model organisms is carried out using both nuclear and cytoplasmic extracts. For a long time, Drosophila’s embryos have represented the main source for protein extractions, although in the last year, the importance of collecting proteins extracts also from larval tissues has also been understood. Here we report a very simple protocol, improved by a previously developed method, to produce in a single extraction both highly stable nuclear and cytoplasmic protein extracts from a small quantity of whole Drosophila’s larvae or tissues, suitable for biochemical analyses like co-immunoprecipitation.
机译:蛋白质和蛋白质在几种模型生物中的功能的鉴定和研究都是使用核和细胞质提取物进行的。长期以来,果蝇的胚胎一直是蛋白质提取的主要来源,尽管在去年,人们也了解了从幼体组织中收集蛋白质提取物的重要性。在这里,我们报告了一种非常简单的方案,该方案通过先前开发的方法进行了改进,可以从少量完整的果蝇幼虫或组织中一次提取出高度稳定的核和胞质蛋白提取物,适用于诸如共免疫沉淀法等生化分析。

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