• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>International journal of infectious diseases : >Usefulness of environmental DNA for detecting Schistosoma mansoni occurrence sites in Madagascar
【24h】

Usefulness of environmental DNA for detecting Schistosoma mansoni occurrence sites in Madagascar

机译：环境DNA在马达加斯加检测曼氏血吸虫发生部位的有用性

获取原文
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Objectives Schistosomiasis is an important disease in Madagascar, and several studies on the disease have focused on the occurrence of the parasite in humans. However, the range of the pathogen in the environment and its impact on human infection is difficult to predict. An environmental DNA (eDNA) detection system for Schistosoma mansoni was developed to improve schistosomiasis eco-epidemiology studies. Methods Primers and probes were designed and tested in experimental biotopes. The field study was conducted in Maevatanana District of Madagascar. Seven water sources with human use were sampled, with a total of 21 water samples collected. Snails were collected, and patients were examined by ultrasound to determine the occurrence of schistosomiasis in the study area. Results One water source with active transmission was identified through the detection of S. mansoni eDNA in the water and the intermediate host Biomphalaria pfeifferi collected from the same water source. People with clinical schistosomiasis were found in the area, reinforcing the findings. Conclusions The application of eDNA in eco-epidemiology enables the determination of hot spots and safe spots in endemic areas, constituting an alternative ecological tool for follow-up and monitoring of control programs for schistosomiasis, and contributing information on water safety for improving the standard of living of the people in endemic areas.
机译：目的血吸虫病是马达加斯加的一种重要疾病，有关该疾病的多项研究集中于人类体内寄生虫的发生。但是，环境中病原体的范围及其对人类感染的影响很难预测。开发了曼氏血吸虫的环境DNA（eDNA）检测系统，以改善血吸虫病的生态流行病学研究。方法设计引物和探针，并在实验生物群落中进行测试。实地研究在马达加斯加的Maevatanana区进行。采样了七个人类使用的水源，总共收集了21个水样。收集蜗牛，并通过超声检查患者，以确定研究区域内血吸虫病的发生。结果通过检测水中曼氏链球菌eDNA和从同一水源收集到的中间宿主Biomphalaria pfeifferi，鉴定出一种主动传播的水源。在该地区发现了具有临床血吸虫病的人，这进一步证实了这一发现。结论eDNA在生态流行病学中的应用可以确定流行地区的热点和安全点，构成了对血吸虫病控制计划进行跟踪和监测的替代生态工具，并为提高水的标准提供了水安全信息。人们在流行地区的生活。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号