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首页> 外文期刊>International Journal of Molecular Sciences >Proteogenomic Characterization of Novel x-Type High Molecular Weight Glutenin Subunit 1Ax1.1
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Proteogenomic Characterization of Novel x-Type High Molecular Weight Glutenin Subunit 1Ax1.1

机译：新型x型高分子量谷蛋白亚基1Ax1.1的蛋白质组学表征。

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Analysis of Portuguese wheat (Triticum aestivum L.) landrace ‘Barbela’ revealed the existence of a new x-type high molecular weight-glutenin subunit (HMW-GS) encoded at the Glu-A1 locus, which we named 1Ax1.1. Using one-dimensional and two-dimensional electrophoresis and mass spectrometry, we compared subunit 1Ax1.1 with other subunits encoded at the Glu-A1 locus. Subunit 1Ax1.1 has a theoretical molecular weight of 93,648 Da (or 91,508 Da for the mature protein) and an isoelectric point (pI) of about 5.7, making it the largest and most acidic HMW-GS known to be encoded at Glu-A1. Specific primers were designed to amplify and sequence 2601 bp of the Glu-A1 locus from the ‘Barbela 28’ wheat genome. A very high level of identity was found between the sequence encoding 1Ax1.1 and those encoding other alleles of the locus. The major difference found was an insertion of 36 amino acids in the central repetitive domain.
机译：对葡萄牙小麦（Triticum aestivum L.）地方品种“ Barbela”的分析显示，存在一个新的x型高分子量谷蛋白亚基（HMW-GS），该基因在Glu-A1位点编码，我们命名为1Ax1.1。使用一维和二维电泳和质谱，我们比较了1Ax1.1亚基和在Glu-A1基因座编码的其他亚基。亚基1Ax1.1的理论分子量为93,648 Da（或成熟蛋白为91,508 Da），等电点（pI）约为5.7，使其成为已知在Glu-A1编码的最大，最酸性的HMW-GS 。设计了特异性引物，以扩增'Barbela 28'小麦基因组中Glu-A1基因座的2601 bp序列。在编码1Ax1.1的序列与编码基因座其他等位基因的序列之间发现了很高的同一性。发现的主要区别是在中央重复结构域中插入了36个氨基酸。

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