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首页> 外文期刊>International Journal of Molecular Sciences >The CYP51F1 Gene of Leptographium qinlingensis: Sequence Characteristic, Phylogeny and Transcript Levels
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The CYP51F1 Gene of Leptographium qinlingensis: Sequence Characteristic, Phylogeny and Transcript Levels

机译：秦岭eptCYP51F1基因的序列特征，系统发生和转录水平

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Leptographium qinlingensis is a fungal associate of the Chinese white pine beetle (Dendroctonus armandi) and a pathogen of the Chinese white pine (Pinus armandi) that must overcome the terpenoid oleoresin defenses of host trees. L. qinlingensis responds to monoterpene flow with abundant mechanisms that include export and the use of these compounds as a carbon source. As one of the fungal cytochrome P450 proteins (CYPs), which play important roles in general metabolism, CYP51 (lanosterol 14-α demethylase) can catalyze the biosynthesis of ergosterol and is a target for antifungal drug. We have identified an L. qinlingensis CYP51F1 gene, and the phylogenetic analysis shows the highest homology with the 14-α-demethylase sequence from Grosmannia clavigera (a fungal associate of Dendroctonus ponderosae). The transcription level of CYP51F1 following treatment with terpenes and pine phloem extracts was upregulated, while using monoterpenes as the only carbon source led to the downregulation of CYP5F1 expression. The homology modeling structure of CYP51F1 is similar to the structure of the lanosterol 14-α demethylase protein of Saccharomyces cerevisiae YJM789, which has an N-terminal membrane helix 1 (MH1) and transmembrane helix 1 (TMH1). The minimal inhibitory concentrations (MIC) of terpenoid and azole fungicides (itraconazole (ITC)) and the docking of terpenoid molecules, lanosterol and ITC in the protein structure suggested that CYP51F1 may be inhibited by terpenoid molecules by competitive binding with azole fungicides.
机译：秦岭ept是中国白松甲虫（Dendroctonus armandi）的真菌同伴，并且是中国白松（Pinus armandi）的病原体，必须克服寄主树的萜类油树脂的防御能力。秦岭落叶松对单萜的流动有多种反应机制，包括出口和将这些化合物用作碳源。 CYP51是一种在一般代谢中起重要作用的真菌细胞色素P450蛋白（CYP），它可以催化麦角固醇的生物合成，是抗真菌药物的靶标。我们已经鉴定出秦岭乳酸菌CYP51F1基因，并且系统发育分析显示与Grosmannia clavigera（Dendroctonus pokerosae的真菌伴）的14-α-脱甲基酶序列具有最高的同源性。用萜类和松树韧皮部提取物处理后，CYP51F1的转录水平上调，而使用单萜类作为唯一碳源导致CYP5F1表达下调。 CYP51F1的同源性建模结构与酿酒酵母YJM789的羊毛甾醇14-α脱甲基酶蛋白的结构相似，其具有N端膜螺旋1（MH1）和跨膜螺旋1（TMH1）。萜类和唑类杀真菌剂（伊曲康唑（ITC））的最小抑制浓度（MIC）以及类萜分子，羊毛甾醇和ITC在蛋白质结构中的对接表明，通过与吡咯类杀真菌剂竞争性结合，萜类分子可能会抑制CYP51F1。

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