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首页> 外文期刊>International journal of infectious diseases : >Molecular epidemiology of enteric viruses and genotyping of rotavirus A, adenovirus and astrovirus among children under 5 years old in Gabon
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Molecular epidemiology of enteric viruses and genotyping of rotavirus A, adenovirus and astrovirus among children under 5 years old in Gabon

机译：加蓬5岁以下儿童肠道病毒的分子流行病学和轮状病毒A，腺病毒和星状病毒的基因分型

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Objectives: This study aimed to determine the prevalence of enteric viruses causing gastroenteritis, and the circulating stains, in Gabonese children under five years old who visited health centers between March 2010 and June 2011. Methods: Stool specimens were collected and sent for analysis to CIRMF (Centre International de Recherches Medicales de Franceville). Stools were screened for six enteric viruses (rotavirus, adenovirus, norovirus I and II, sapovirus, human astrovirus) by means of a multiplex real-time reverse transcription polymerase chain reaction, and Rotavirus A, Adenovirus and Astrovirus were genotyped. Results: Among the 317 specimens analyzed, 193 (60.9%) were positive for at least one enteric virus. Rotavirus A (RVA) (27.1%) was the most frequently detected virus, followed by human Adenovirus (HAdV) (19.6%), Norovirus II (NoVs-II) (13.9%), Norovirus I (NoVs-I) (9.1%), Sapovirus (SaV) (9.5%) and human Astrovirus (HAstV) (6.3%). One-third of the 193 positive samples contained more than one virus. The most common Rotavirus A genotype was G6P[6]. Various HAdV serotypes were found. HAstV-1 was identified. Conclusions: These findings improve our knowledge of circulating enteric viruses in Gabon. The emergence of unusual G6P[6] strain of rotavirus A, predominant, suggested a particular epidemiological surveillance of circulating rotavirus strains during the introduction of vaccination in Gabon.
机译：目的：本研究旨在确定2010年3月至2011年6月在加蓬访问卫生中心的五岁以下加蓬儿童中引起肠胃炎的肠道病毒的流行率和循环污点。方法：收集粪便样本并送至CIRMF分析（法国国际医学研究中心）。通过多重实时逆转录聚合酶链反应，对凳子筛选了六种肠病毒（轮状病毒，腺病毒，诺和病毒I和II，Sapovirus，人星状病毒），并对轮状病毒A，腺病毒和星状病毒进行了基因分型。结果：在分析的317个样本中，有193个（60.9％）至少一种肠病毒呈阳性。轮状病毒A（RVA）（27.1％）是最常见的病毒，其次是人腺病毒（HAdV）（19.6％），诺如病毒II（NoVs-II）（13.9％），诺如病毒I（NoVs-I）（9.1％）病毒，Sapovirus（SaV）（9.5％）和人星状病毒（HAstV）（6.3％）。 193个阳性样本中有三分之一包含一种以上的病毒。最常见的轮状病毒A基因型是G6P [6]。发现了各种HAdV血清型。鉴定出HAstV-1。结论：这些发现提高了我们对加蓬循环肠道病毒的认识。主要是轮状病毒A的异常G6P [6]菌株的出现，表明在加蓬接种疫苗期间对循环轮状病毒株进行了特殊的流行病学监测。

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