Isolation and Characterization of 11 New Microsatellite Loci in Erigeron breviscapus (Asteraceae), an Important Chinese Traditional Herb

Junbo Yang; Kexian Song; Tingshuang Yi; Xiang Li

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Isolation and Characterization of 11 New Microsatellite Loci in Erigeron breviscapus (Asteraceae), an Important Chinese Traditional Herb

机译：重要中草药灯盏花新的11个新微卫星基因座的分离与鉴定

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Erigeron breviscapus (Vant.) Hand.-Mazz. (Asteraceae) is a species endemic to southwestern China and an important traditional Chinese herb for cardiovascular and cerebral vessel diseases. Applying a modified biotin-streptavidin capture method, 11 microsatellite loci were discovered. Polymorphism of each locus was assessed in 24 individuals collected from five wild populations. The number of alleles per locus ranged from 2 to 7, with an average of 4.273. The observed (HO) and expected (HE) heterozygosities varied from 0.250 to 0.958 and from 0.337 to 0.786, respectively. Over half of these loci were successfully amplified in two congeneric species. The developed microsatellite markers will be useful for future population genetics and conservation studies, as well as accurate identification of different varieties.

机译：灯盏花灯（手放）-马兹。菊科（Asteraceae）是中国西南地区特有的物种，是治疗心血管和脑血管疾病的重要传统中草药。应用改进的生物素-链霉亲和素捕获方法，发现了11个微卫星基因座。在从五个野生种群中收集的24个个体中评估了每个基因座的多态性。每个基因座的等位基因数量范围为2到7，平均为4.273。观察到的（HO）和预期的（HE）杂合度分别为0.250至0.958和0.337至0.786。这些基因座的一半以上在两个同类物种中成功扩增。开发的微卫星标记将对未来的种群遗传学和保存研究以及准确鉴定不同品种有用。

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《International Journal of Molecular Sciences》 |2011年第10期|共6页
作者
Junbo Yang; Kexian Song; Tingshuang Yi; Xiang Li;
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中图分类生物物理学;
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6. Isolation and Characterization of 11 New Microsatellite Loci in Erigeron breviscapus (Asteraceae) an Important Chinese Traditional Herb [O] . Xiang Li, Kexian Song, Junbo Yang, 2011

机译：重要中草药灯盏花11个新的微卫星基因座的分离与鉴定
7. Isolation and Characterization of 11 New Microsatellite Loci in Erigeron breviscapus (Asteraceae), an Important Chinese Traditional Herb [O] . Li, Xiang, Song, Kexian, Yang, Junbo, 2011

机译：重要中草药灯盏花11个新的微卫星基因座的分离与鉴定

Isolation and Characterization of 11 New Microsatellite Loci in Erigeron breviscapus (Asteraceae), an Important Chinese Traditional Herb

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