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首页> 外文期刊>International Journal of Molecular Sciences >Mechanistic Investigation of ROS-Induced DNA Damage by Oestrogenic Compounds in Lymphocytes and Sperm Using the Comet Assay
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Mechanistic Investigation of ROS-Induced DNA Damage by Oestrogenic Compounds in Lymphocytes and Sperm Using the Comet Assay

机译：彗星试验研究淋巴细胞和精子中致雌性化合物对ROS诱导的DNA损伤的机理

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Past research has demonstrated that oestrogenic compounds produce strand breaks in the DNA of sperm and lymphocytes via reactive oxygen species (ROS). In the current investigation, sperm and lymphocytes were treated in vitro with oestrogenic compounds (diethylstilboestrol, progesterone, 17β-oestradiol, noradrenaline and triiodotyronine) and several aspects of DNA damage were investigated. Firstly, mediation of DNA damage by lipid peroxidation was investigated in the presence of BHA (a lipid peroxidation blocker). BHA reduced the DNA damage generated by 17β-oestradiol and diethylstilboestrol in a statistically significant manner. No effects were observed for sperm. Secondly, the presence of oxidized bases employing FPG and EndoIII were detected for lymphocytes and sperm in the negative control and after 24 h recovery in lymphocytes but not immediately after treatment for both cell types. The successful detection of oxidized bases in the negative control (untreated) of sperm provides an opportunity for its application in biomonitoring studies. DNA repair at 24 h after exposure was also studied. A nearly complete recovery to negative control levels was shown in lymphocytes 24 h recovery after oestrogenic exposure and this was statistically significant in all cases. Rapid rejoining of DNA, in a matter of hours, is a characteristic of DNA damaged by ROS.
机译：过去的研究表明，雌激素化合物可通过活性氧（ROS）在精子和淋巴细胞的DNA中产生链断裂。在目前的研究中，用雌激素化合物（二乙基雌二醇，孕酮，17β-雌二醇，去甲肾上腺素和三碘代酪氨酸）体外处理精子和淋巴细胞，并研究了DNA损伤的几个方面。首先，研究了在BHA（脂质过氧化阻滞剂）存在下脂质过氧化对DNA损伤的介导作用。 BHA以统计学上显着的方式减少了17β-雌二醇和二乙基雌二醇产生的DNA损伤。没有观察到对精子的影响。其次，在阴性对照中以及在淋巴细胞中恢复24小时后，在淋巴细胞和精子中检测到存在使用FPG和EndoIII氧化的碱基，但在两种细胞类型处理后均未立即检测到。成功检测出阴性对照（未处理）的精子中的氧化碱基，为将其应用于生物监测研究提供了机会。还研究了暴露后24小时的DNA修复。暴露于雌激素后24小时，淋巴细胞恢复到阴性对照水平，几乎完全恢复，并且在所有情况下均具有统计学意义。在短短几个小时内，DNA快速重新结合是ROS破坏DNA的特征。

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