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首页> 外文期刊>International Journal of Molecular Sciences >Development of a Modular Assay for Detailed Immunophenotyping of Peripheral Human Whole Blood Samples by Multicolor Flow Cytometry
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Development of a Modular Assay for Detailed Immunophenotyping of Peripheral Human Whole Blood Samples by Multicolor Flow Cytometry

机译：通过多色流式细胞术对周围人全血样本进行详细免疫分型的模块化检测方法的开发

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The monitoring of immune cells gained great significance in prognosis and prediction of therapy responses. For analyzing blood samples, the multicolor flow cytometry has become the method of choice as it combines high specificity on single cell level with multiple parameters and high throughput. Here, we present a modular assay for the detailed immunophenotyping of blood (DIoB) that was optimized for an easy and direct application in whole blood samples. The DIoB assay characterizes 34 immune cell subsets that circulate the peripheral blood including all major immune cells such as T cells, B cells, natural killer (NK) cells, monocytes, dendritic cells (DCs), neutrophils, eosinophils, and basophils. In addition, it evaluates their functional state and a few non-leukocytes that also have been associated with the outcome of cancer therapy. This DIoB assay allows a longitudinal and close-meshed monitoring of a detailed immune status in patients requiring only 2.0 mL of peripheral blood and it is not restricted to peripheral blood mononuclear cells. It is currently applied for the immune monitoring of patients with glioblastoma multiforme (IMMO-GLIO-01 trial, NCT02022384), pancreatic cancer (CONKO-007 trial, NCT01827553), and head and neck cancer (DIREKHT trial, NCT02528955) and might pave the way for immune biomarker identification for prediction and prognosis of therapy outcome.
机译：免疫细胞的监测在预后和治疗反应的预测中具有重要意义。对于分析血液样本，多色流式细胞术已成为一种选择的方法，因为它结合了单细胞水平的高特异性与多个参数和高通量。在这里，我们介绍了一种用于详细血液免疫表型（DIoB）的模块化测定方法，该方法针对全血样品中的简单直接应用进行了优化。 DIoB分析表征了循环血液的34个免疫细胞亚群，包括所有主要免疫细胞，例如T细胞，B细胞，自然杀伤（NK）细胞，单核细胞，树突状细胞（DC），嗜中性粒细胞，嗜酸性粒细胞和嗜碱性粒细胞。此外，它评估了它们的功能状态以及一些与癌症治疗结果相关的非白细胞。这种DIoB测定法可对仅需要2.0 mL外周血的患者进行详细的免疫状态的纵向和密网监测，并且不限于外周血单核细胞。它目前用于多形性胶质母细胞瘤（IMMO-GLIO-01试验，NCT02022384），胰腺癌（CONKO-007试验，NCT01827553）和头颈癌（DIREKHT试验，NCT02528955）患者的免疫监测，可能为免疫生物标志物鉴定的方法，可预测和预测治疗结果。

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