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首页> 外文期刊>International Journal of Molecular Sciences >Isolation and Characterization of Microsatellite Loci in the Chinese Cobra Naja atra (Elapidae)
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Isolation and Characterization of Microsatellite Loci in the Chinese Cobra Naja atra (Elapidae)

机译：中国眼镜蛇眼镜蛇（天牛）微卫星基因座的分离与鉴定

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摘要

We characterize thirteen polymorphic microsatellite loci isolated from Naja atra genomic libraries, which were enriched for AC-motif microsatellites. The thirteen loci were screened on a group of 48 individuals from two populations, one in Yong’an and the other in Ganzhou. These markers revealed a relatively high degree of genetic diversity (4–12 alleles per locus) and heterozygosity (Ho ranged from 0.213–0.854 and He ranged from 0.301–0.838). Tests for departure from Hardy-Weinberg equilibrium and for linkage disequilibrium were conducted for each of the two populations separately. After sequential Bonferroni correction, none of the 13 loci showed significant departures from Hardy-Weinberg equilibrium. Hierarchical analysis of molecular variance indicated that a small but significant (P 0.001) proportion (16.0%) of the total variation in the microsatellite DNA data were attributable to differences among populations, indicating geographical structuring and restricted gene flow. It could be attributable to the Wuyi mountains in the area having a sufficiently isolating effect to significantly reduce gene flow. Our microsatellite data also showed a low Nm (1.31) value in the two populations from mainland China. Thus, the Yong’an and Ganzhou populations could be treated as distinct evolutionarily significant units (ESUs). The high level of polymorphism revealed by these microsatellite markers will be useful for the study of gene flow, population structure and evolutionary history of N. atra.
机译：我们表征了从眼镜蛇Atra基因组库中分离出的13个多态微卫星基因座，这些基因库富含AC型微卫星。在来自两个种群的48个个体中筛选了13个基因座，一个在永安，另一个在甘州。这些标记物揭示了相对较高的遗传多样性（每个基因座4–12个等位基因）和杂合性（Ho介于0.213–0.854之间，He介于0.301–0.838之间）。分别对两个种群分别进行了偏离哈迪-温伯格平衡和链接不平衡的检验。经过连续的Bonferroni校正后，这13个位点均未显示出与Hardy-Weinberg平衡显着偏离。分子变异的分层分析表明，微卫星DNA数据的总变异中有一个很小但显着（P <0.001）的比例（16.0％）可归因于种群之间的差异，表明地理结构和基因流受限。这可能归因于该地区的武夷山具有足够的隔离作用，从而大大减少了基因流。我们的微卫星数据还显示，来自中国大陆的两个种群的N m （1.31）值较低。因此，永安和赣州人口可以被视为不同的进化重要单位（ESU）。这些微卫星标记揭示的高水平多态性将有助于研究背叶猪笼草的基因流动，种群结构和进化史。

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