...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>International Journal of Molecular Sciences >Gold-Coated Iron Composite Nanospheres Targeted the Detection of Escherichia coli
【24h】

Gold-Coated Iron Composite Nanospheres Targeted the Detection of Escherichia coli

机译：镀金铁复合纳米球靶向大肠杆菌的检测

获取原文
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

We report the preparation and characterization of spherical core-shell structured Fe3O4–Au magnetic nanoparticles, modified with two component self-assembled monolayers (SAMs) consisting of 3–mercaptophenylboronic acid (3–MBA) and 1–decanethiol (1–DT). The rapid and room temperature synthesis of magnetic nanoparticles was achieved using the hydroxylamine reduction of HAuCl4 on the surface of ethylenediaminetetraacetic acid (EDTA)-immobilized iron (magnetite Fe3O4) nanoparticles in the presence of an aqueous solution of hexadecyltrimetylammonium bromide (CTAB) as a dispersant. The reduction of gold on the surface of Fe3O4 nanoparticles exhibits a uniform, highly stable, and narrow particle size distribution of Fe3O4–Au nanoparticles with an average diameter of 9 ± 2 nm. The saturation magnetization value for the resulting nanoparticles was found to be 15 emu/g at 298 K. Subsequent surface modification with SAMs against glucoside moieties on the surface of bacteria provided effective magnetic separation. Comparison of the bacteria capturing efficiency, by means of different molecular recognition agents 3–MBA, 1–DT and the mixed monolayer of 3–MBA and 1–DT was presented. The best capturing efficiency of E. coli was achieved with the mixed monolayer of 3–MBA and 1–DT-modified nanoparticles. Molecular specificity and selectivity were also demonstrated by comparing the surface-enhanced Raman scattering (SERS) spectrum of E. coli-nanoparticle conjugates with bacterial growth media.
机译：我们报告了球形核-壳结构的Fe 3 O 4 –Au磁性纳米粒子的制备和表征，该纳米粒子经两组分自组装单分子膜（SAMs）改性，该分子由3–巯基苯硼酸（3-MBA）和1-癸硫醇（1-DT）。通过在乙二胺四乙酸（EDTA）固定铁（磁铁矿Fe 3 O <的表面）上的HAuCl 4 的羟胺还原反应实现了磁性纳米粒子的快速室温合成sub> 4 ）纳米颗粒在十六烷基三甲基溴化铵（CTAB）水溶液中作为分散剂存在。 Fe 3 O 4 纳米颗粒表面的金还原表现出均匀，高度稳定且窄的Fe 3 O粒度分布 4 –Au纳米粒子，平均直径为9±2 nm。发现所得纳米颗粒的饱和磁化强度值为298 K时为15 emu / g。随后用SAM对细菌表面的糖苷部分进行表面改性可提供有效的磁分离效果。介绍了通过不同的分子识别剂3-MBA，1-DT和3-MBA和1-DT的混合单层细菌捕获效率的比较。 3–MBA和1–DT修饰的纳米颗粒的混合单层可以实现最佳的大肠杆菌捕获效率。通过比较大肠杆菌-纳米颗粒结合物与细菌生长培养基的表面增强拉曼散射（SERS）光谱，也证明了分子特异性和选择性。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号