...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>International Journal of Molecular Sciences >Neuropeptide Substance-P-Conjugated Chitosan Nanofibers as an Active Modulator of Stem Cell Recruiting
【24h】

Neuropeptide Substance-P-Conjugated Chitosan Nanofibers as an Active Modulator of Stem Cell Recruiting

机译：神经肽物质-P-结合的壳聚糖纳米纤维作为干细胞募集的主动调节剂。

获取原文
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

The goal to successful wound healing is essentially to immobilize and recruit appropriate numbers of host stem or progenitor cells to the wound area. In this study, we developed a chitosan nanofiber-immobilized neuropeptide substance-P (SP), which mediates stem cell mobilization and migration, onto the surfaces of nanofibers using a peptide-coupling agent, and evaluated its biological effects on stem cells. The amount of immobilized SP on chitosan nanofibers was modulated over the range of 5.89 ± 3.27 to 75.29 ± 24.31 ng when reacted with 10 to 500 ng SP. In vitro migration assays showed that SP-incorporated nanofibers induced more rapid migration of human mesenchymal stem cells on nanofibers compared to pristine samples. Finally, the conjugated SP evoked a minimal foreign body reaction and recruited a larger number of CD29- and CD44-positive stem cells into nanofibers in a mouse subcutaneous pocket model.
机译：成功伤口愈合的目标实质上是将适当数量的宿主干细胞或祖细胞固定并募集到伤口区域。在这项研究中，我们开发了一种壳聚糖纳米纤维固定的神经肽物质-P（SP），该物质使用肽偶联剂介导干细胞的动员和迁移到纳米纤维的表面，并评估了其对干细胞的生物学作用。当与10至500 ng SP反应时，固定在壳聚糖纳米纤维上的SP量在5.89±3.27至75.29±24.31 ng范围内调节。体外迁移试验表明，与原始样品相比，掺有SP的纳米纤维诱导人间充质干细胞在纳米纤维上迁移更快。最后，缀合的SP引起最小的异物反应，并在小鼠皮下口袋模型中将大量的CD29和CD44阳性干细胞募集到纳米纤维中。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号