...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>International Journal of Molecular Sciences >A Novel VHH Antibody Targeting the B Cell-Activating Factor for B-Cell Lymphoma
【24h】

A Novel VHH Antibody Targeting the B Cell-Activating Factor for B-Cell Lymphoma

机译：针对B细胞淋巴瘤的B细胞活化因子的新型VHH抗体

获取原文
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Objective: To construct an immune alpaca phage display library, in order to obtain a single domain anti-BAFF (B cell-activating factor) antibody. Methods: Using phage display technology, we constructed an immune alpaca phage display library, selected anti-BAFF single domain antibodies (sdAbs), cloned three anti-BAFF single-domain antibody genes into expression vector pSJF2, and expressed them efficiently in Escherichia coli. The affinity of different anti-BAFF sdAbs were measured by Bio layer interferometry. The in vitro biological function of three sdAbs was investigated by cell counting kit-8 (CCK-8) assay and a competitive enzyme-linked immunosorbent assay (ELISA). Results: We obtained three anti-BAFF single domain antibodies (anti-BAFF64, anti-BAFF52 and anti-BAFFG3), which were produced in high yield in Escherichia coli and inhibited tumor cell proliferation in vitro. Conclusion: The selected anti-BAFF antibodies could be candidates for B-cell lymphoma therapies.
机译：目的：构建免疫羊驼噬菌体展示文库，以获得单结构域抗BAFF（B细胞活化因子）抗体。方法：利用噬菌体展示技术，构建免疫羊驼噬菌体展示文库，选择抗BAFF单域抗体（sdAbs），将三个抗BAFF单域抗体基因克隆到表达载体pSJF2中，并在大肠杆菌中高效表达。通过生物层干涉术测量不同抗BAFF sdAb的亲和力。通过细胞计数试剂盒8（CCK-8）测定和竞争性酶联免疫吸附测定（ELISA）研究了三种sdAb的体外生物学功能。结果：我们获得了三种抗BAFF单结构域抗体（anti-BAFF64，anti-BAFF52和anti-BAFFG3），它们在大肠杆菌中高产，并能在体外抑制肿瘤细胞的增殖。结论：选择的抗BAFF抗体可作为B细胞淋巴瘤治疗的候选药物。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号