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首页> 外文期刊>International Journal of Molecular Sciences >Antibacterial Activity of Ciprofloxacin-Encapsulated Cockle Shells Calcium Carbonate (Aragonite) Nanoparticles and Its Biocompatability in Macrophage J774A.1
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Antibacterial Activity of Ciprofloxacin-Encapsulated Cockle Shells Calcium Carbonate (Aragonite) Nanoparticles and Its Biocompatability in Macrophage J774A.1

机译：环丙沙星包裹的鸟蛤壳碳酸钙（文石）纳米颗粒的抗菌活性及其在巨噬细胞J774A.1中的生物相容性。

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The use of nanoparticle delivery systems to enhance intracellular penetration of antibiotics and their retention time is becoming popular. The challenge, however, is that the interaction of nanoparticles with biological systems at the cellular level must be established prior to biomedical applications. Ciprofloxacin–cockle shells-derived calcium carbonate (aragonite) nanoparticles (C-CSCCAN) were developed and characterized. Antibacterial activity was determined using a modified disc diffusion protocol on Salmonella Typhimurium ( S. Typhimurium). Biocompatibilittes with macrophage were evaluated using the 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) and 5-Bromo-2′-deoxyuridine (BrdU) assays. Transcriptional regulation of interleukin 1 beta (IL-1β) was determined using reverse transcriptase-polymerase chain reaction (RT-PCR). C-CSCCAN were spherical in shape, with particle sizes ranging from 11.93 to 22.12 nm. Encapsulation efficiency (EE) and loading content (LC) were 99.5% and 5.9%, respectively, with negative ζ potential. X-ray diffraction patterns revealed strong crystallizations and purity in the formulations. The mean diameter of inhibition zone was 18.6 ± 0.5 mm, which was better than ciprofloxacin alone (11.7 ± 0.9 mm). Study of biocompatability established the cytocompatability of the delivery system without upregulation of IL-1β. The results indicated that ciprofloxacin–nanoparticles enhanced the antibacterial efficacy of the antibiotic, and could act as a suitable delivery system against intracellular infections.
机译：使用纳米颗粒递送系统来增强抗生素在细胞内的渗透及其保留时间变得很普遍。然而，挑战在于必须在生物医学应用之前建立纳米颗粒与生物系统在细胞水平上的相互作用。环丙沙星-蛤壳衍生的碳酸钙（文石）纳米颗粒（C-CSCCAN）得到了开发和表征。使用改良的椎间盘扩散协议对鼠伤寒沙门氏菌（鼠伤寒沙门氏菌）测定抗菌活性。使用3-（4,5-二甲基噻唑-2-基）-2,5-二苯基溴化四溴化铵（MTT）和5-溴2'-脱氧尿苷（BrdU）分析评估了具有巨噬细胞的生物相容性。使用逆转录聚合酶链反应（RT-PCR）确定白介素1β（IL-1β）的转录调控。 C-CSCCAN为球形，粒径范围为11.93至22.12 nm。包封效率（EE）和负载含量（LC）分别为99.5％和5.9％，ζ电位为负。 X-射线衍射图显示制剂中强烈的结晶和纯度。抑制区的平均直径为18.6±0.5 mm，优于单独的环丙沙星（11.7±0.9 mm）。生物相容性研究建立了输送系统的细胞相容性，而没有上调IL-1β。结果表明，环丙沙星纳米颗粒增强了抗生素的抗菌功效，并且可以作为抵抗细胞内感染的合适传递系统。

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