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首页> 外文期刊>International Journal of Molecular Sciences >Involvement of Three Esterase Genes from Panonychus citri (McGregor) in Fenpropathrin Resistance
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Involvement of Three Esterase Genes from Panonychus citri (McGregor) in Fenpropathrin Resistance

机译：泛酸柑橘中的三种酯酶基因与苯丙氨酸酯抗性有关

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The citrus red mite, Panonychus citri (McGregor), is a major citrus pest with a worldwide distribution and an extensive record of pesticide resistance. However, the underlying molecular mechanism associated with fenpropathrin resistance in this species have not yet been reported. In this study, synergist triphenyl phosphate (TPP) dramatically increased the toxicity of fenpropathrin, suggesting involvement of carboxylesterases (CarEs) in the metabolic detoxification of this insecticide. The subsequent spatiotemporal expression pattern analysis of PcE1 , PcE7 and PcE9 showed that three CarEs genes were all over-expressed after insecticide exposure and higher transcripts levels were observed in different field resistant strains of P. citri . Heterologous expression combined with 3-(4,5-dimethyl-thiazol-2-yl)-2,5-diphenyltetra-zolium bromide (MTT) cytotoxicity assay in Spodoptera frugiperda (Sf9) cells revealed that PcE1 -, PcE7 - or PcE9 -expressing cells showed significantly higher cytoprotective capability than parental Sf9 cells against fenpropathrin, demonstrating that PcEs probably detoxify fenpropathrin. Moreover, gene silencing through the method of leaf-mediated dsRNA feeding followed by insecticide bioassay increased the mortalities of fenpropathrin-treated mites by 31% ( PcE1 ), 27% ( PcE7 ) and 22% ( PcE9 ), respectively, after individual PcE gene dsRNA treatment. In conclusion, this study provides evidence that PcE1 , PcE7 and PcE9 are functional genes mediated in fenpropathrin resistance in P. citri and enrich molecular understanding of CarEs during the resistance development of the mite.
机译：柑橘红螨Panonychus citri（McGregor）是一种主要的柑橘害虫，在世界范围内分布并且对杀虫剂的耐受性广泛。然而，尚未报道与该物种中的苯丙酸菊酯抗性有关的潜在分子机制。在这项研究中，协同增效剂磷酸三苯酯（TPP）显着增加了苯丙酸菊酯的毒性，表明羧酸酯酶（CarEs）参与了该杀虫剂的代谢解毒。随后对PcE1，PcE7和PcE9进行时空表达模式分析表明，在暴露于杀虫剂后，三个CarEs基因全部过表达，并且在不同的田间抗性柠檬果蝇菌株中观察到更高的转录水平。异源表达与3-（4,5-二甲基噻唑-2-基）-2,5-二苯基四唑溴化物（MTT）在草地贪夜蛾（Sf9）细胞中的细胞毒性实验表明，PcE1-，PcE7-或PcE9-表达表达的细胞显示出比亲本Sf9细胞更高的抗苯丙草酸的细胞保护能力，表明PcE可能使苯丙草胺解毒。此外，通过叶介导的dsRNA喂养方法进行基因沉默后再进行杀虫剂生物测定，分别将单个原发性大肠杆菌基因处理后的螨病死亡率分别提高了31％（PcE1），27％（PcE7）和22％（PcE9）。 dsRNA处理。总而言之，这项研究提供了证据，证明PcE1，PcE7和PcE9是在柑橘黄粉病中对苯甲酚原抗性介导的功能基因，并在螨的抗性发展过程中丰富了对CarEs的分子理解。

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