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首页> 外文期刊>International Journal of Molecular Sciences >Gene-Transformation-Induced Changes in Chemical Functional Group Features and Molecular Structure Conformation in Alfalfa Plants Co-Expressing Lc - bHLH and C1-MYB Transcriptive Flavanoid Regulatory Genes: Effects of Single-Gene and Two-Gene Insertion
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Gene-Transformation-Induced Changes in Chemical Functional Group Features and Molecular Structure Conformation in Alfalfa Plants Co-Expressing Lc - bHLH and C1-MYB Transcriptive Flavanoid Regulatory Genes: Effects of Single-Gene and Two-Gene Insertion

机译：基因转化诱导的苜蓿植物中共表达Lc-bHLH和C1-MYB转录类黄酮调控基因的化学官能团特征和分子结构构型变化：单基因和两基因插入的影响

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Alfalfa ( Medicago sativa L.) genotypes transformed with Lc-bHLH and Lc transcription genes were developed with the intention of stimulating proanthocyanidin synthesis in the aerial parts of the plant. To our knowledge, there are no studies on the effect of single-gene and two-gene transformation on chemical functional groups and molecular structure changes in these plants. The objective of this study was to use advanced molecular spectroscopy with multivariate chemometrics to determine chemical functional group intensity and molecular structure changes in alfalfa plants when co-expressing Lc-bHLH and C1-MYB transcriptive flavanoid regulatory genes in comparison with non-transgenic (NT) and AC Grazeland (ACGL) genotypes. The results showed that compared to NT genotype, the presence of double genes ( Lc and C1 ) increased ratios of both the area and peak height of protein structural Amide I/II and the height ratio of α-helix to β-sheet. In carbohydrate-related spectral analysis, the double gene-transformed alfalfa genotypes exhibited lower peak heights at 1370, 1240, 1153, and 1020 cm ?1 compared to the NT genotype. Furthermore, the effect of double gene transformation on carbohydrate molecular structure was clearly revealed in the principal component analysis of the spectra. In conclusion, single or double transformation of Lc and C1 genes resulted in changing functional groups and molecular structure related to proteins and carbohydrates compared to the NT alfalfa genotype. The current study provided molecular structural information on the transgenic alfalfa plants and provided an insight into the impact of transgenes on protein and carbohydrate properties and their molecular structure’s changes.
机译：开发了用Lc-bHLH和Lc转录基因转化的苜蓿（紫花苜蓿）基因型，目的是刺激植物地上部分原花青素的合成。据我们所知，目前尚无关于单基因和两基因转化对这些植物化学官能团和分子结构变化的影响的研究。这项研究的目的是使用先进的分子光谱技术和多元化学计量学方法，确定与非转基因（NT）一起共表达Lc-bHLH和C1-MYB转录类黄酮调节基因时，苜蓿植物的化学官能团强度和分子结构变化）和AC Grazeland（ACGL）基因型。结果表明，与NT基因型相比，双重基因（Lc和C1）的存在增加了蛋白质结构酰胺I / II的面积和峰高比以及α-螺旋与β-折叠的高度比。在与碳水化合物有关的光谱分析中，与NT基因型相比，经双基因转化的苜蓿基因型在1370、1240、1153和1020cm？1处显示出较低的峰高。此外，在光谱的主成分分析中清楚地揭示了双基因转化对碳水化合物分子结构的影响。总之，与NT苜蓿基因型相比，Lc和C1基因的单或双转化导致与蛋白质和碳水化合物有关的官能团和分子结构改变。当前的研究提供了有关转基因苜蓿植物的分子结构信息，并提供了对转基因对蛋白质和碳水化合物特性及其分子结构变化的影响的见解。

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