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首页> 外文期刊>International Journal of Molecular Sciences >Early Transcriptomic Response to Phosphate Deprivation in Soybean Leaves as Revealed by RNA-Sequencing
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Early Transcriptomic Response to Phosphate Deprivation in Soybean Leaves as Revealed by RNA-Sequencing

机译：RNA测序显示对大豆叶片中磷酸盐缺乏的早期转录组学反应

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摘要

Low phosphate (Pi) availability is an important limiting factor affecting soybean production. However, the underlying molecular mechanisms responsible for low Pi stress response and tolerance remain largely unknown, especially for the early signaling events under low Pi stress. Here, a genome-wide transcriptomic analysis in soybean leaves treated with a short-term Pi-deprivation (24 h) was performed through high-throughput RNA sequencing (RNA-seq) technology. A total of 533 loci were found to be differentially expressed in response to Pi deprivation, including 36 mis-annotated loci and 32 novel loci. Among the differentially expressed genes (DEGs), 303 were induced and 230 were repressed by Pi deprivation. To validate the reliability of the RNA-seq data, 18 DEGs were randomly selected and analyzed by quantitative RT-PCR (reverse transcription polymerase chain reaction), which exhibited similar fold changes with RNA-seq. Enrichment analyses showed that 29 GO (Gene Ontology) terms and 8 KEGG (Kyoto Encyclopedia of Genes and Genomes) pathways were significantly enriched in the up-regulated DEGs and 25 GO terms and 16 KEGG pathways were significantly enriched in the down-regulated DEGs. Some DEGs potentially involved in Pi sensing and signaling were up-regulated by short-term Pi deprivation, including five SPX-containing genes. Some DEGs possibly associated with water and nutrient uptake, hormonal and calcium signaling, protein phosphorylation and dephosphorylation and cell wall modification were affected at the early stage of Pi deprivation. The cis-elements of PHO (phosphatase) element, PHO-like element and P responsive element were present more frequently in promoter regions of up-regulated DEGs compared to that of randomly-selected genes in the soybean genome. Our transcriptomic data showed an intricate network containing transporters, transcription factors, kinases and phosphatases, hormone and calcium signaling components is involved in plant responses to early Pi deprivation.
机译：磷酸盐（Pi）利用率低是影响大豆产量的重要限制因素。然而，导致低Pi胁迫反应和耐受的潜在分子机制仍然是未知的，特别是对于低Pi胁迫下的早期信号事件。在这里，通过高通量RNA测序（RNA-seq）技术对短期Pi剥夺（24 h）处理的大豆叶片进行了全基因组转录组分析。发现总共533个基因座响应Pi剥夺而差异表达，包括36个错误注释的基因座和32个新的基因座。在差异表达基因（DEGs）中，Pi剥夺诱导了303个基因并抑制了230个基因。为了验证RNA-seq数据的可靠性，随机选择了18个DEG并通过定量RT-PCR（逆转录聚合酶链反应）进行了分析，这些RNA表现出与RNA-seq相似的倍数变化。富集分析表明，上调的DEG中显着富集了29个GO（基因本体论）术语和8个KEGG（遗传基因组京都百科全书）途径，而下调的DEG中显着富集了25个GO术语和16个KEGG途径。一些可能参与Pi感测和信号传导的DEGs被短期Pi剥夺上调，包括五个含有SPX的基因。在剥夺Pi的早期阶段，一些可能与水分和养分吸收，激素和钙信号传导，蛋白质磷酸化和去磷酸化以及细胞壁修饰有关的DEG受到影响。与大豆基因组中随机选择的基因相比，PHO（磷酸酶）元件，PHO样元件和P响应元件的顺式元件在上调DEGs的启动子区域中的出现频率更高。我们的转录组数据显示，包含转运蛋白，转录因子，激酶和磷酸酶，激素和钙信号传导成分的复杂网络参与了植物对早期Pi剥夺的反应。

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