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首页> 外文期刊>Investigative ophthalmology & visual science >Corneal Biomechanical Properties from Two-Dimensional Corneal Flap Extensiometry: Application to UV-Riboflavin Cross-Linking
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Corneal Biomechanical Properties from Two-Dimensional Corneal Flap Extensiometry: Application to UV-Riboflavin Cross-Linking

机译：二维角膜瓣拉伸技术测定角膜生物力学特性：在紫外-核黄素交联中的应用

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Purpose.: Corneal biomechanical properties are usually measured by strip extensiometry or inflation methods. We developed a two-dimensional (2D) flap extensiometry technique, combining the advantages of both methods, and applied it to measure the effect of UV-Riboflavin cross-linking (CXL). Methods.: Corneal flaps (13 pig/8 rabbit) from the de-epithelialized anterior stroma (96 ??m) were mounted on a custom chamber, consisting of a BK7 lens, a reflective retina, and two reservoirs (filled with Riboflavin and silicone oil). Stretching the corneal flap during five pressure increase/decrease cycles (0a??30 mm Hg) changed the refractive power of the system, whose Zernike aberrations were monitored with a ray-tracing aberrometer. Porcine flaps were used to test the system. Rabbits were treated with CXL unilaterally in vivo following standard clinical procedures. Flaps were measured 1 month postoperatively. An analytical model allowed estimating Young's modulus from the change in surface (strain) and pressure (stress). Confocal microscopy examination was performed before, and at different times after CXL. Results.: Flap curvature changed with increased function of IOP in pig flaps (23.4 ?? 10a??3 D/mm Hg). In rabbit flaps curvature changed significantly less in 1 month post CXL (P = 0.026) than in untreated corneas [17.0 vs. 6.36 millidiopter (mD)/mm Hg]. Young's modulus was 2.29 megapascals (MPa) in porcine corneas, 1.98 MPa in untreated rabbit corneas, and 4.83 MPa in 1 month post CXL rabbit corneas. At the same time, highly reflective structures were observed in the rabbit midstroma after treatment. Conclusions.: 2D flap extensiometry allows estimating corneal elasticity in vitro. The measurements are spatially resolved in depth, minimize the effects of corneal hydration, and preserve the integrity of the cornea. The method proved the efficacy of CXL in increasing corneal rigidity after 1 month in rabbits.
机译：目的：角膜的生物力学性能通常通过带状拉伸或充气方法进行测量。我们结合了这两种方法的优点，开发了二维（2D）皮瓣引伸技术，并将其应用于测量UV-核黄素交联（CXL）的效果。方法：将来自上皮化的前基质（96？m）的角膜瓣（13头猪/ 8只兔子）安装在定制腔室中，该腔室由BK7晶状体，反射性视网膜和两个储液槽（充满核黄素和硅油）。在五个压力增加/减少周期（0a≤30mm Hg）中拉伸角膜瓣，改变了系统的屈光力，并通过光线跟踪像差仪监测了其Zernike像差。猪皮瓣用于测试系统。按照标准临床程序，在体内用CXL单面治疗兔子。术后1个月测量皮瓣。一个分析模型可以根据表面（应变）和压力（应力）的变化估算杨氏模量。共聚焦显微镜检查在CXL之前和之后进行。结果：在猪皮瓣中，皮瓣曲率随IOP功能的增加而改变（23.4 ?? 10a ?? 3 D / mm Hg）。在CXL后1个月，兔皮瓣曲率变化明显小于未治疗的角膜[17.0对6.36毫屈光度（mD）/ mm Hg]。猪角膜的杨氏模量为2.29兆帕（MPa），未经处理的兔子角膜的杨氏模量为1.98 MPa，CXL兔子角膜术后1个月的杨氏模量为4.83 MPa。同时，在处理后的兔中基质中观察到高反射结构。结论：2D皮瓣引伸术可以估计体外角膜弹性。测量在深度上在空间上解析，最大程度地减少了角膜水化的影响，并保留了角膜的完整性。该方法证明了CXL在提高兔子1个月后角膜刚度方面的功效。

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