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首页> 外文期刊>Investigative ophthalmology & visual science >Electrospun nanofiber matrices for corneal tissue engineering: Comparison of the introduced linker groups in Polycaprolactone fibers blended with chitosan and Poly(glycerol sebacate)
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Electrospun nanofiber matrices for corneal tissue engineering: Comparison of the introduced linker groups in Polycaprolactone fibers blended with chitosan and Poly(glycerol sebacate)

机译：用于角膜组织工程的电纺纳米纤维基质：聚己内酯纤维与壳聚糖和聚癸二酸甘油酯共混物中引入的接头基团的比较

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Purpose : We have previously shown that Polycaprolactone (PCL) / Poly(glycerol sebacate) (PGS) nanofiber matrices can be modified by introduction of surface linker groups. To evaluate alternative materials, we now blended PCL with chitosan, a polymer that already contains the surface linker groups and compared the amounts of these. Methods : Nanofiber matrices of PCL were produced by electrospinning. These meshes consisted of PCL alone, of PCL blended with chitosan and of PCL blended with PGS. The fiber meshes have been cut into circles with a diameter of 12 mm. PCL and PCL/PGS samples were functionalized in a first step by a wet-chemical treatment, while the chitosan containing samples stayed untreated. During this step amino-functional groups were introduced to the ester-groups of the PCL fibers. In the second step all samples, also the chitosan containing ones, were treated to bind thio-functional groups to the amino-groups. By use of the Ellmana??s reagent the amount of introduced thio-functional groups could be measured. After the modification human corneal epithelial cells (HCEC) have been incubated on the modified material and the biological activity of the cells was measured by cell bioactivity assay (WST-8) to detect any effect of the modification on the cells. Results : Thiol groups could be introduced by the described wet-chemical process in PCL alone [20.25 ?± 6,59 nM] and PCL/PGS [24,78 ?± 9,78 nM] samples. The Ellmana??s assay showed also the existence of amino-functional groups on the surface of PCL/chitosan [23,59 ?± 4,88 nM] samples. SEM images did not show major changes in fiber morphology as a result of the wet-chemical treatment. The WST-8 assay showed no significant changes in activity of the cultured cells on treated and untreated materials. Conclusions : Introduction of functional linker groups on the surface of PCL and PCL/PGS were possible, whereas a similar amount of functional-amino groups existed already in the PCL/chitosan fibers. No negative effects of the treatment could be detected, whether checking the fiber morphology by SEM or the cell activity by WST-8 assay. Further steps will show how binding of active molecules to the fiber meshes will improve our material for the tissue engineering. This is an abstract that was submitted for the 2016 ARVO Annual Meeting, held in Seattle, Wash., May 1-5, 2016.
机译：目的：我们先前已经证明，可以通过引入表面连接基团来修饰聚己内酯（PCL）/聚癸二酸甘油酯（PGS）纳米纤维基质。为了评估替代材料，我们现在将PCL与壳聚糖共混，壳聚糖是一种已经包含表面连接基团的聚合物，并比较了它们的含量。方法：通过电纺丝法制备PCL纳米纤维基质。这些网由单独的PCL，掺有壳聚糖的PCL和掺有PGS的PCL组成。将纤维网切成直径为12 mm的圆形。第一步，通过湿化学处理对PCL和PCL / PGS样品进行功能化，而含壳聚糖的样品则未经处理。在该步骤中，将氨基官能团引入PCL纤维的酯基。在第二步中，处理所有样品，也包括含壳聚糖的样品，以将硫官能团与氨基结合。通过使用Ellmana的试剂，可以测量引入的硫官能团的量。修饰的人角膜上皮细胞（HCEC）在修饰的材料上孵育后，通过细胞生物活性测定（WST-8）测量细胞的生物学活性，以检测修饰对细胞的任何作用。结果：硫醇基团可通过上述湿化学方法引入PCL [20.25±6.59 nM]和PCL / PGS [24.78±9.78 nM]样品中。 Ellmana的分析表明在PCL /壳聚糖[23,59？±4,88 nM]样品的表面上也存在氨基官能团。 SEM图像没有显示出通过湿化学处理的纤维形态的主要变化。 WST-8分析表明，在处理过的和未处理过的材料上，培养细胞的活性没有明显变化。结论：可以在PCL和PCL / PGS表面引入功能性连接基团，而PCL /壳聚糖纤维中已经存在相似数量的功能性氨基。无论是通过SEM检查纤维形态还是通过WST-8分析检测细胞活性，均未检测到治疗的负面影响。进一步的步骤将显示出活性分子与纤维网的结合将如何改善我们用于组织工程的材料。这是提交给2016年5月1-5日在华盛顿州西雅图市举行的2016 ARVO年会的摘要。

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