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首页> 外文期刊>Investigative ophthalmology & visual science >Active Maintenance of the Gradient of Refractive Index Is Required to Sustain the Optical Properties of the Lens
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Active Maintenance of the Gradient of Refractive Index Is Required to Sustain the Optical Properties of the Lens

机译：要保持镜片的光学特性，需要积极保持折射率梯度的保持

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Purpose: To determine whether the cellular physiology of the lens actively maintains the optical properties of the lens and whether inhibition of lens transport affects overall visual quality. Methods: One lens from a pair of bovine lenses was cultured in artificial aqueous humor (AAH), while the other was cultured in either AAH-High-K+ or AAH + 0.1 mM ouabain for 4 hours. Lens pairs or whole enucleated eyes were then imaged in 4.7 Tesla (T) high-field small animal magnet. Lens surface curvatures, T1 measurements of water content, and T2 measurements of water/protein ratios were extracted from cultured lenses, while the geometrical parameters that define the optical pathway were obtained from whole eyes. Gradients of refractive index (GRIN), calculated from T2 measurements, and the extracted geometric parameters were inputted into optical models of the isolated lens and the whole bovine eye. Results: Inhibiting circulating fluxes by inhibiting the Na/K-ATPase with ouabain or depolarization of the lens potential by High K+ caused changes to lens water content, the water/protein ratio (GRIN) and surface geometry that manifested as an increase in optical power and a decrease in negative spherical aberration in cultured lenses. Changes to optical properties of the lens resulted in a myopic shift that impaired vision quality in the optical model of the bovine eye. Conclusions: The cellular physiology of the lens actively maintains its optical properties and inhibiting the Na/K/ATPase induces a myopic shift in vision similar to that observed clinically in patients who go on to develop cataract.
机译：目的：确定晶状体的细胞生理学是否能积极维持晶状体的光学特性，以及抑制晶状体运输是否会影响整体视觉质量。方法：将一对牛晶状体中的一个晶状体在人工房水（AAH）中培养，将另一个晶状体在AAH-High-K +或AAH + 0.1 mM哇巴因中培养4小时。然后，在4.7特斯拉（T）高场小动物磁铁中拍摄成对的眼镜或全无核的眼睛。从培养的镜片中提取镜片的表面曲率，水分含量的T1测量值和水/蛋白质比的T2测量值，而从整个眼睛中获得定义光路的几何参数。由T2测量值计算得出的折射率梯度（GRIN）和提取的几何参数被输入到孤立的晶状体和整个牛眼的光学模型中。结果：通过哇巴因抑制Na / K-ATPase来抑制循环通量或高K +使晶状体电位去极化，导致晶状体含水量，水/蛋白质比（GRIN）和表面几何形状发生变化，表现为光焦度增加并减少了人工晶状体的负球差。晶状体光学特性的变化导致近视移位，从而损害了牛眼光学模型的视觉质量。结论：晶状体的细胞生理学可以积极地维持其光学特性，并且抑制Na / K / ATPase会引起近视视力改变，类似于继续发展为白内障患者的临床观察。

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