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National Tuberculosis Genotyping and Surveillance Network: Design and Methods

机译:全国结核病基因分型和监测网络:设计和方法

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The National Tuberculosis Genotyping and Surveillance Network was established in 1996 to perform a 5-year, prospective study of the usefulness of genotyping Mycobacterium tuberculosis isolates to tuberculo-sis control programs. Seven sentinel sites identified all new cases of tuberculosis, collected information onpatients and contacts, and obtained patient isolates. Seven genotyping laboratories performed DNA finger-printing analysis by the international standard IS6110 method. BioImage Whole Band Analyzer softwarewas used to analyze patterns, and distinct patterns were assigned unique designations. Isolates with six orfewer bands on IS6110 patterns were also spoligotyped. Patient data and genotyping designations wereentered in a relational database and merged with selected variables from the national surveillance data-base. In two related databases, we compiled the results of routine contact investigations and the results ofinvestigations of the relationships of patients who had isolates with matching genotypes. We describe themethods used in the study
机译:国家结核病基因分型和监测网络成立于1996年,进行了为期5年的前瞻性研究,目的是对结核分枝杆菌菌株进行基因分型对结核病控制计划的实用性。七个哨点确定了所有新的结核病病例,收集了有关患者和接触者的信息,并获得了患者分离株。七个基因分型实验室按照国际标准IS6110方法进行了DNA指纹分析。使用BioImage全波段分析仪软件分析模式,并为不同的模式分配唯一的名称。在IS6110模式上具有六个或更少条带的分离株也被定型。将患者数据和基因分型名称输入到关系数据库中,并与来自国家监视数据库的选定变量合并。在两个相关的数据库中,我们汇总了常规接触调查的结果以及具有分离的具有匹配基因型的患者的关系的调查结果。我们描述了研究中使用的方法

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