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首页> 外文期刊>Emerging Infectious Diseases >Rapid Typing of Transmissible Spongiform Encephalopathy Strains with Differential ELISA
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Rapid Typing of Transmissible Spongiform Encephalopathy Strains with Differential ELISA

机译：差异ELISA快速鉴定可传播的海绵状脑病菌株

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The bovine spongiform encephalopathy (BSE) agent has been transmitted to humans, leading to variant Creutzfeldt-Jakob disease. Sheep and goats can be experi-mentally infected by BSE and have been potentially exposed to natural BSE; however, whether BSE can be transmitted to small ruminants is not known. Based on the particular bio-chemical properties of the abnormal prion protein (PrPsc) as-sociated with BSE, and particularly the increased degrada-tion induced by proteinase K in the N terminal part of PrPsc, we have developed a rapid ELISA designed to distinguish BSE from other scrapie strains. This assay clearly discrimi-nates experimental ovine BSE from other scrapie strains and was used to screen 260 transmissible spongiform encephal-opathy (TSE)–infected small ruminant samples identifi ed by the French active surveillance network (2002/2003). In this context, this test has helped to identify the fi rst case of natural BSE in a goat and can be used to classify TSE isolates based on the proteinase K sensitivity of PrPsc
机译：牛海绵状脑病（BSE）病原已传播给人类，导致变异型克雅氏病。绵羊和山羊可能在实验中被BSE感染，并可能暴露于天然BSE中。但是，尚不清楚是否可以将BSE传播给小型反刍动物。基于与疯牛病相关的异常病毒蛋白（PrPsc）的特殊生化特性，尤其是蛋白酶K引起的PrPsc N末端降解的增加，我们开发了一种快速ELISA试剂盒，用于区分来自其他瘙痒病菌株的疯牛病。该测定法清楚地将实验羊疯牛病与其他瘙痒病菌株区分开来，并用于筛选由法国主动监视网络（2002/2003）鉴定的260例传染性海绵状脑病（TSE）感染的小反刍动物样品。在这种情况下，该测试有助于鉴定山羊中天然BSE的第一例，并可用于根据PrPsc的蛋白酶K敏感性对TSE分离株进行分类

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