...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Emerging Infectious Diseases >Detection of SARS-associated Coronavirus in Throat Wash and Saliva in Early Diagnosis
【24h】

Detection of SARS-associated Coronavirus in Throat Wash and Saliva in Early Diagnosis

机译：早期诊断喉咙和唾液中与SARS相关的冠状病毒的检测

获取原文
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

The severe acute respiratory syndrome–associatedcoronavirus (SARS-CoV) is thought to be transmitted pri-marily through dispersal of droplets, but little is knownabout the load of SARS-CoV in oral droplets. We examinedoral specimens, including throat wash and saliva, andfound large amounts of SARS-CoV RNA in both throatwash (9.58 x 102to 5.93 x 106copies/mL) and saliva (7.08x 103to 6.38 x 108copies/mL) from all specimens of 17consecutive probable SARS case-patients, supporting thepossibility of transmission through oral droplets.Immunofluorescence study showed replication of SARS-CoV in the cells derived from throat wash, demonstratingthe possibility of developing a convenient antigen detectionassay. This finding, with the high detection rate a median of4 days after disease onset and before the development oflung lesions in four patients, suggests that throat wash andsaliva should be included in sample collection guidelinesfor SARS diagnosis.
机译：严重的急性呼吸系统综合症相关冠状病毒（SARS-CoV）被认为主要是通过飞沫的传播而传播，但对SARS-CoV在口服飞沫中的负荷了解甚少。我们检查了包括喉咙冲洗液和唾液在内的口腔标本，并从17份可能的SARS的所有标本中分别在喉咙冲洗液（9.58 x 102至5.93 x 106份/ mL）和唾液（7.08x 103至6.38 x 108份/ mL）中发现了大量SARS-CoV RNA免疫荧光研究表明SARS-CoV在咽喉冲洗液来源的细胞中复制，证明了开发便利的抗原检测方法的可能性。这一发现具有很高的发现率，在四名患者发病后至肺部病变发生之前的中位检测时间为4天，这表明应将咽喉冲洗液和唾液纳入SARS诊断的样本收集指南中。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号