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Serologic and Molecular Biologic Methods for SARS-associated Coronavirus Infection, Taiwan

机译：台湾SARS相关冠状病毒感染的血清学和分子生物学方法

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Severe acute respiratory syndrome (SARS) has raiseda global alert since March 2003. After its causative agent,SARS-associated coronavirus (SARS-CoV), was con-firmed, laboratory methods, including virus isolation,reverse transcriptase–polymerase chain reaction (RT-PCR), and serologic methods, have been quickly devel-oped. In this study, we evaluated four serologic tests ( neu-tralization test, enzyme-linked immunosorbent assay[ELISA], immunofluorescent assay [IFA], and immunochro-matographic test [ICT]) for detecting antibodies to SARS-CoV in sera of 537 probable SARS case-patients with cor-relation to the RT-PCR . With the neutralization test as areference method, the sensitivity, specificity, positive pre-dictive value, and negative predictive value were 98.2%,98.7%, 98.7%, and 98.4% for ELISA; 99.1%, 87.8%, 88.1%and 99.1% for IFA; 33.6%, 98.2%, 95.7%, and 56.1% forICT, respectively. We also compared the recombinant-based western blot with the whole virus–based IFA andELISA; the data showed a high correlation between thesemethods, with an overall agreement of >90%. Our resultsprovide a systematic analysis of serologic and molecularmethods for evaluating SARS-CoV infection
机译：自2003年3月以来，严重急性呼吸综合征（SARS）引起了全球关注。在确认其病原体为SARS相关冠状病毒（SARS-CoV）之后，实验室方法包括病毒分离，逆转录酶-聚合酶链反应（RT） -PCR）和血清学方法已迅速开发。在这项研究中，我们评估了四种血清学检测（中和检测，酶联免疫吸附测定[ELISA]，免疫荧光测定[IFA]和免疫色谱测定[ICT]），以检测537血清中的SARS-CoV抗体与RT-PCR相关的SARS病例患者。以中和试验为参考方法，ELISA的灵敏度，特异性，阳性预测值和阴性预测值分别为98.2％，98.7％，98.7％和98.4％。 IFA为99.1％，87.8％，88.1％和99.1％; ICT分别为33.6％，98.2％，95.7％和56.1％。我们还比较了基于重组的蛋白质印迹和基于病毒的IFA和ELISA。数据显示这些方法之间具有高度相关性，总体一致性> 90％。我们的结果为评估SARS-CoV感染提供了血清学和分子方法的系统分析

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