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Co-infections in Visceral Pentastomiasis, Democratic Republic of the Congo

机译:刚果民主共和国内脏性肺囊炎的合并感染

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Snakeborne Armillifer pentastomiasis is an emerging human parasitic infection in rural tropical areas where snake meat is eaten. After a series of severe ocular A. grandis larval infections and anecdotal abdominal infection in Sankuru District, Democratic Republic of the Congo, during 2014–2015, we systematically investigated possible pentastomid etiology in patients who underwent surgery in the region. Histologic and molecular analyses by established pentastomid 18S rDNA- and newly developed Armillifer -specific cytochrome oxidase PCRs revealed larval pentastomid lesions in 3.7% of patients. Some persons had A. armillatus and A. grandis co-infections. Another pentastomid larva, Raillietiella sp., was molecularly detected in 1 patient who had concomitant A. grandis and A. armillatus infection. The PCRs used were suitable for detecting pentastomid species even in highly necrotic tissues. Phylogenetic analyses of Armillifer cytochrome oxidase genes detected multiple local strains.
机译:Snakeborne Armillifer pentastomiasis是食用蛇肉的热带热带地区的一种新型人类寄生虫感染。在2014年至2015年期间,在刚果民主共和国桑库鲁区发生了一系列严重的眼大曲霉幼虫感染和腹部轶事感染之后,我们系统地调查了该地区接受手术的患者中可能存在的戊五mid病病因。组织学和分子分析已通过建立的五stomid 18S rDNA和新开发的Armillifer特异的细胞色素氧化酶PCR进行检测,发现3.7%的患者存在幼虫pentostomid病变。有些人患有圆曲霉和大曲霉共同感染。在另一名同时感染大曲霉和蜜曲霉的患者中,发现了另一种五味胺幼虫Raillietiella sp.。所用的PCR试剂盒甚至在高度坏死的组织中也适用于检测戊糖酰胺类。系统发育分析的Armillifer细胞色素氧化酶基因检测到多个本地菌株。

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