...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Emerging Infectious Diseases >Infectious Schmallenberg Virus from Bovine Semen, Germany
【24h】

Infectious Schmallenberg Virus from Bovine Semen, Germany

机译：德国牛精液中的传染性Schmallenberg病毒

获取原文
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Commercially produced semen straws with egg yolk–based diluent were used for the injection of 6- to 9-month-old heifers. The straws originated from 6 semen batches (quantification cycle [Cq] values 26.4–36.4) collected from 6 bulls (designated A–C and E–G) during August and September 2012 (1). To increase the probability of SBV infection of injected cattle, 5 straws of semen (≈220 μL each) from 1 batch from an individual bull were pooled and diluted in minimal essential medium with antibiotics to 4 mL. Six cattle (C1–6) were subcutaneously inoculated, each with a pool from 1 of the 6 bulls. To investigate the infectivity of a single insemination dose (1 straw), 5 cattle (C7–C11) were subcutaneously injected with single straws from bull F that had been confirmed to contain infectious SBV. Serum samples were obtained on several days (Figure), and clinical signs and rectal body temperatures for the injected cattle were monitored daily.
机译：商业生产的精液秸秆与蛋黄基稀释剂一起用于注射6到9个月大的小母牛。稻草来自2012年8月和2012年9月从6头公牛（指定为A–C和E–G）收集的6批精液（量化周期[C q ]值26.4–36.4）（1）。为了增加被注射牛的SBV感染的可能性，将来自单个公牛的1批中的5根精液秸秆（每个≈220μL）汇集起来，并在含抗生素的基本必需培养基中稀释至4 mL。皮下接种了六头牛（C1-6），每头牛均来自6头公牛中的1头。为了研究单次授精剂量（1根吸管）的传染性，对5头牛（C7–C11）皮下注射了来自公牛F的单根吸管，这些吸管已被证实含有传染性SBV。在几天内获得血清样品（图），并每天监测注射牛的临床体征和直肠体温。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号