...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Genetics: A Periodical Record of Investigations Bearing on Heredity and Variation >DNA SEQUENCE COMPARISON AMONG CLOSELY RELATED DROSOPHILA SPECIES IN THE MULLERI COMPLEX
【24h】

DNA SEQUENCE COMPARISON AMONG CLOSELY RELATED DROSOPHILA SPECIES IN THE MULLERI COMPLEX

机译：MULLERI复合体中近缘果蝇属物种的DNA序列比较

获取原文
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

DNA hybridization was used to establish DNA sequence relationships among seven Drosophila species. Single-copy DNA was isolated from four species within the Drosophila mulleri complex, D. mojavensis, D. arizonensis, D. ritae and D. starmeri . These single-copy DNAs were used as tracers to be hybridized with each other and one additional member of the mulleri complex, D. aldrichi , a member of a closely related complex, D. hydei , and a distantly related species, D. melanogaster . Two methods have been used to determine the relatedness between these species: (1) the extent of duplex formed as measured by binding to hydroxyapatite and (2) the thermal stability of the duplexed DNA. Moderately repetitive DNA was purified from these species and used similarly to determine the divergence of this family of sequences. The rate of nucleotide substitution was estimated to be 0.2 ± 0.1% base pair change per million years for both single-copy and middle-repetitive DNAs. The size of the D. arizonensis genome, a representative of the mulleri complex, was calculated to be 2.2 × 108 base pairs from its kinetic complexity similar to that of D. hydei . The relative amounts (18%) and average reiteration frequency (100 copies) of the middle-repetitive DNA are similar for all Drosophila species studied. Finally, the data are presented in a phylogenetic tree.
机译：DNA杂交用于建立7种果蝇之间的DNA序列关系。从果蝇复合体，D。mojavensis，D。arizonensis，D。ritae和D. starmeri内的四个物种中分离出单拷贝DNA。这些单拷贝DNA用作示踪剂，可与mulleri复合体的另外一个成员D. aldrichi，密切相关的复合体D. hydei的成员以及远缘近缘种D. melanogaster杂交。已经使用两种方法来确定这些物质之间的相关性：（1）通过结合羟基磷灰石测量的双链体形成程度;（2）双链体DNA的热稳定性。从这些物种中纯化出适度重复的DNA，并类似地用于确定该序列家族的差异。对于单拷贝和中等重复DNA，核苷酸取代率估计为每百万年0.2±0.1％碱基对变化。根据其动力学复杂性，类似于海藻（D. hydei），计算出了穆勒氏菌复合体代表的亚利桑那海藻（D. arizonensis）基因组的大小为2.2×108个碱基对。对于所有研究的果蝇物种，中等重复DNA的相对含量（18％）和平均重复频率（100拷贝）相似。最后，数据在系统发育树中显示。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号