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首页> 外文期刊>Genetics: A Periodical Record of Investigations Bearing on Heredity and Variation >Temperature-sensitive mutations that cause stage-specific defects in Zebrafish fin regeneration.
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Temperature-sensitive mutations that cause stage-specific defects in Zebrafish fin regeneration.

机译：对温度敏感的突变会导致斑马鱼鳍再生的特定阶段缺陷。

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摘要

When amputated, the fins of adult zebrafish rapidly regenerate the missing tissue. Fin regeneration proceeds through several stages, including wound healing, establishment of the wound epithelium, recruitment of the blastema from mesenchymal cells underlying the wound epithelium, and differentiation and outgrowth of the regenerate. We screened for temperature-sensitive mutations that affect the regeneration of the fin. Seven mutations were identified, including five that fail to regenerate their fins, one that causes slow growth during regeneration, and one that causes dysmorphic bumps or tumors to develop in the regenerating fin. reg5 mutants fail to regenerate their caudal fins, whereas reg6 mutants develop dysmorphic bumps in their regenerates at the restrictive temperature. Temperature-shift experiments indicate that reg5 and reg6 affect different stages of regeneration. The critical period for reg5 occurs during the early stages of regeneration before or during establishment of the blastema, resulting in defects in subsequent growth of the blastema and failure to differentiate bone-forming cells. The critical period for reg6 occurs after the onset of bone differentiation and during early stages of regenerative outgrowth. Both reg5 and reg6 also show temperature-sensitive defects in embryonic development or in ontogenetic outgrowth of the juvenile fin.
机译：当被截肢时，成年斑马鱼的鳍迅速再生缺失的组织。鳍的再生经历多个阶段，包括伤口愈合，伤口上皮的建立，伤口上皮下的间充质细胞募集胚细胞，以及再生的分化和长出。我们筛选了影响鳍片再生的温度敏感突变。确定了七个突变，包括五个无法使鳍再生的突变，一个导致再生过程中生长缓慢的突变，以及一个导致变形的突起或肿瘤在再生鳍中发育的突变。 reg5突变体无法再生其尾鳍，而reg6突变体在限定温度下其再生物中会产生畸形的颠簸。温移实验表明reg5和reg6影响再生的不同阶段。 reg5的关键期发生在胚泡形成之前或过程中的再生早期，从而导致胚泡随后生长中的缺陷以及无法分化成骨细胞。 reg6的关键时期发生在骨分化开始后和再生长出早期。 reg5和reg6在幼鱼的胚胎发育或个体发育中也显示出对温度敏感的缺陷。

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