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首页> 外文期刊>Genetics: A Periodical Record of Investigations Bearing on Heredity and Variation >ELEVATED LEVELS OF PETITE FORMATION IN STRAINS OF SACCHAROMYCES CEREVISIAE RESTORED TO RESPIRATORY COMPETENCE. I. ASSOCIATION OF BOTH HIGH AND MODERATE FREQUENCIES OF PETITE MUTANT FORMATION WITH THE PRESENCE OF ABERRANT MITOCHONDRIAL DNA
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ELEVATED LEVELS OF PETITE FORMATION IN STRAINS OF SACCHAROMYCES CEREVISIAE RESTORED TO RESPIRATORY COMPETENCE. I. ASSOCIATION OF BOTH HIGH AND MODERATE FREQUENCIES OF PETITE MUTANT FORMATION WITH THE PRESENCE OF ABERRANT MITOCHONDRIAL DNA

机译：恢复了呼吸能力的糖酵母酿酒酵母菌株中的小体积PET形成。 I.突变体形成的高中度频率与线粒体DNA异常的关联

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When recently arisen spontaneous petite mutants of Saccharomyces cerevisiae are crossed, respiratory competent diploids can be recovered. Such restored strains can be divided into two groups having sectored or unsectored colony morphology, the former being due to an elevated level of spontaneous petite mutation. On the basis of petite frequency, the sectored strains can be subdivided into those with a moderate frequency (5-16%) and those with a high frequency (60%) of petite formation. Each of the three categories of restored strains can be found on crossing two petites, suggesting either that the parental mutants contain a heterogeneous population of deleted mtDNAs at the time of mating or that different interactions can occur between the defective molecules. Restriction endonuclease analysis of mtDNA from restored strains that have a wild-type petite frequency showed that they had recovered a wild-type mtDNA fragmentation pattern. Conversely, all examined cultures from both categories of sectored strains contained aberrant mitochondrial genomes that were perpetuated without change over at least 200 generations. In addition, sectored colony siblings can have different aberrant mtDNAs. The finding that two sectored, restored strains from different crosses have identical but aberrant mtDNAs provides evidence for preferred deletion sites from the mitochondrial genome. Although it appears that mtDNAs from sectored strains invariably contain duplications, there is no apparent correlation between the size of the duplication and spontaneous petite frequency.
机译：当最近出现的酿酒酵母的自发小身材突变体杂交时，可以恢复呼吸功能二倍体。这种恢复的菌株可以分为具有扇形或不扇形的菌落形态的两组，前者是由于自发性娇小突变水平升高所致。根据小频率，可以将扇形应变细分为中等频率（5-16％）和高频（> 60％）小频率形成。可以在穿越两个小宠物时发现三种恢复菌株中的每一种，这表明在交配时亲代突变体包含缺失的mtDNA的异质群体，或者缺陷分子之间可能发生不同的相互作用。从具有野生型小频率的恢复菌株中对mtDNA进行限制性核酸内切酶分析表明，它们已恢复了野生型mtDNA片段化模式。相反，来自两类扇形菌株的所有检测培养物中都含有异常的线粒体基因组，这些基因组在至少200代中没有发生改变。此外，扇形的殖民地兄弟姐妹可以具有不同的异常mtDNA。来自不同杂交的两个扇形，恢复的菌株具有相同但异常的mtDNA的发现，为线粒体基因组中优选的缺失位点提供了证据。尽管看起来来自扇形菌株的mtDNA总是包含重复项，但重复大小与自发小频率之间没有明显的相关性。

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