首页> 外文期刊>Genetics: A Periodical Record of Investigations Bearing on Heredity and Variation >Isolation of a second S-locus-related cDNA from Brassica oleracea: genetic relationships between the S locus and two related loci.
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Isolation of a second S-locus-related cDNA from Brassica oleracea: genetic relationships between the S locus and two related loci.

机译:从甘蓝中分离第二个与S基因座相关的cDNA:S基因座和两个相关基因座之间的遗传关系。

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Self-incompatibility in Brassica oleracea is controlled by the highly polymorphic S locus. Isolation and subsequent characterization of the S-locus-glycoprotein (SLG) gene, which encodes the S-locus-specific glycoprotein (SLSG), has revealed the presence of a self-incompatibility multigene family. One of these S-locus-related genes, SLR1, has been shown to be expressed. In this study we present the isolation and preliminary characterization of a second expressed S-locus-related sequence, SLR2. Through restriction fragment length polymorphism (RFLP) linkage analysis we demonstrate that the SLR1 and SLR2 loci reside approximately 18.5 map units apart in one linkage group that segregates independently of the S-locus. The identification of a second SLR gene expressed in stigmas suggests that loci unlinked to the S-locus may play a role in the self-incompatibility response, or in pollination in general.
机译:甘蓝型油菜的自交不亲和性由高度多态的S基因座控制。编码S-基因座特异性糖蛋白(SLSG)的S-基因座糖蛋白(SLG)基因的分离和后续表征表明存在自我不相容的多基因家族。这些S-基因座相关基因之一SLR1已被表达出来。在这项研究中,我们介绍了第二个表达的S基因座相关序列SLR2的分离和初步表征。通过限制性片段长度多态性(RFLP)连锁分析,我们证明SLR1和SLR2基因座位于一个独立于S基因座的连锁组中,大约间隔18.5个图单元。对第二个以柱头表达的SLR基因的鉴定表明,未与S-基因座连接的基因座可能在自交不亲和反应或总体授粉中起作用。

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