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首页> 外文期刊>Genetics: A Periodical Record of Investigations Bearing on Heredity and Variation >Mutations in the SPT4, SPT5, and SPT6 Genes Alter Transcription of a Subset of Histone Genes in Saccharomyces cerevisiae
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Mutations in the SPT4, SPT5, and SPT6 Genes Alter Transcription of a Subset of Histone Genes in Saccharomyces cerevisiae

机译：SPT4，SPT5和SPT6基因中的突变改变了酿酒酵母中组蛋白基因的子集的转录。

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摘要

The SPT4, SPT5 , and SPT6 gene products define a class of transcriptional repressors in Saccharomyces cermisiae that are thought to function through their effects on chromatin assembly or stability. Mutations in these genes confer a similar range of phenotypes to mutations in HIR genes, which encode transcriptional repressors that regulate expression of many of the core histone genes. Here we show that mutations in the three SPTgenes also affect transcription of the histone genes that reside at the HTA1-HTB1 locus. HTA1-lacZ transcription was reduced in each spt mutant background, an effect that required a negative site in the HTA1 promoter. The transcriptional effect could be reversed by the overproduction of histones H2A and H2B in an spt4 mutant and histones H3 and H4 in all three spt mutants. Suppression of the spt4 transcriptional defect was dependent on the overproduction of both histones H2A and H2B, and required the presence of N-terminal amino acids in both histones. The results are consistent with the idea that the effects of the spt mutations on nucleosome assembly and/or stability activate repressors of HTA1 transcription.
机译：SPT4，SPT5和SPT6基因产物定义了酿酒酵母中的一类转录阻遏物，被认为是通过它们对染色质组装或稳定性的作用来发挥作用的。这些基因中的突变与HIR基因中的突变具有相似的表型范围，后者编码调节许多核心组蛋白基因表达的转录阻遏物。在这里，我们表明三个SPTgenes中的突变也影响驻留在HTA1-HTB1位点的组蛋白基因的转录。在每个spt突变体背景中，HTA1-lacZ转录均降低，该作用需要HTA1启动子中有一个负位点。在spt4突变体中过高的组蛋白H2A和H2B以及在所有三个spt突变体中过高的组蛋白H3和H4可以逆转转录作用。 spt4转录缺陷的抑制取决于组蛋白H2A和H2B的过量生产，并且需要在两个组蛋白中都存在N末端氨基酸。结果与以下想法一致：spt突变对核小体装配和/或稳定性的影响激活了HTA1转录的阻遏物。

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