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首页> 外文期刊>E3S Web of Conferences >Development of a Novel Fiber Optic Sensor Combined with a Fluorescence Turn-on Probe for Cu (II) Detection
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Development of a Novel Fiber Optic Sensor Combined with a Fluorescence Turn-on Probe for Cu (II) Detection

机译：新型光纤传感器与荧光开启探针的结合，用于Cu（II）检测

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Existing staining-based methodology for the detection of metal ions is not well suited for real-time or in situ use. This is a significant problem, given that these ions can have a considerable impact on both human health and the environment. Thus, there is growing interest and need for simple, rapid and in-situ monitoring techniques for the purpose of detecting various target analytes (e.g. heavy metals), which is of a significant importance in many fields ranging from environmental monitoring to the study of intracellular processes. Among various sensors developed, optical fiber-optic sensors (FOS), based on fluorescence, are one class of sensors that address this goal [1]. Optical fibers are ideal for environmental sensing applications because of their ability to transmit optical signals to and from the sensing region without the use of free-space optics. In this work, we present, for the first time, a simple FOS incorporating novel fluorescence turn-on mechanism [2] that could detect Cu (II) as low as 10?4 M. Traditionally, fluorescence quenching or “turn-off” was used to detect Cu (II) [3]. In recent years, fluorescence “turn-on” emerges as a preferable tool. The developed fiber-optic sensor has two fiber leads and one probe head. One fiber lead includes 6 fibers for He-Ne laser excitation light delivery (e-fibers). Another fiber lead has one receiving fiber (r-fiber) connected to an Ocean Optics QE65000 scientific grade spectrometer, which is interrogated by a computer via USB connection. The SpectroSuite software is used to observe and to record all spectra. The probe head combines all fibers together to form a coaxial structure with the r-fiber placed in the center. The key component in the proposed fluorescent sensing system is a probe prepared by binding a receptor containing a zwitterionic chromophore (M1), through noncovalent interactions, to the fluorescent polymer (P1) resulting in quenching its emission. The sensing mechanism involves the release of P1 (a fluorescent indicator) from its non-fluorescent indicator/receptor (P1/M1) pair upon exposure to Cu (II). The zwitterionic chromophore (M1) was found to show high selectivity and sensitivity to Cu (II), making it an ideal receptor for the recognition of Cu (II) [4]. The optimum operating conditions and performance characteristics for the new sensor will be discussed.Key words: fiber optic sensor / fluorescence turn-on probe / copper
机译：现有的基于染色的用于检测金属离子的方法不适用于实时或原位使用。鉴于这些离子可能对人体健康和环境产生重大影响，因此这是一个重大问题。因此，出于检测各种目标分析物（例如重金属）的目的，对简单，快速和原位监测技术的兴趣日益增长，并且需要这种技术，这在从环境监测到细胞内研究的许多领域中都具有重要意义。流程。在开发的各种传感器中，基于荧光的光纤传感器（FOS）是解决这一目标的一类传感器[1]。光纤是环境感应应用的理想选择，因为它们能够在不使用自由空间光学器件的情况下，将光信号往返于感应区域进行传输。在这项工作中，我们首次展示了一个结合了新型荧光开启机制的简单FOS [2]，该机制可以检测低至10？4 M的Cu（II）。传统上，荧光猝灭或“关闭”用于检测铜（II）[3]。近年来，荧光“开启”逐渐成为一种首选工具。开发的光纤传感器具有两根光纤导线和一个探头。一根光纤导线包括6根用于He-Ne激光激发光传输的光纤（e光纤）。另一根光纤导线的一根接收光纤（r-fiber）连接到Ocean Optics QE65000科学级光谱仪，该计算机通过USB连接对其进行询问。 SpectroSuite软件用于观察和记录所有光谱。探头将所有光纤组合在一起，以将r光纤置于中心形成同轴结构。提出的荧光传感系统中的关键组件是通过非共价相互作用将包含两性离子发色团（M1）的受体与荧光聚合物（P1）结合，从而终止其发射而制备的探针。感应机制涉及在暴露于Cu（II）时从其非荧光指示剂/受体（P1 / M1）对释放P1（一种荧光指示剂）。发现两性离子发色团（M1）对Cu（II）具有很高的选择性和灵敏度，使其成为识别Cu（II）的理想受体[4]。将讨论新型传感器的最佳工作条件和性能特性。关键词：光纤传感器/荧光开启探针/铜

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