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首页> 外文期刊>Molecular and Cellular Biology >Naturally arising recombinants that are missing portions of the simian virus 40 regulatory region.
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Naturally arising recombinants that are missing portions of the simian virus 40 regulatory region.

机译：缺少猿猴病毒40调控区部分的天然产生的重组体。

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When simian virus 40 (SV40) is serially passaged at high multiplicity, a heterogeneous collection of naturally arising variants is generated. Those which are the most abundant presumably have a selective replicative advantage over other defective and wild-type helper SV40s. Two such naturally arising host-substituted variants of SV40 have been characterized in terms of complete nucleotide sequence determination. Evolutionary variant ev-1101 (previously isolated by Lee et al., Virology 66:53-69, 1975) is from undiluted serial passage 13, whereas ev-2101 is newly isolated from undiluted serial passage 6 of an independently-derived evolutionary series. Both variants contain a five-times tandemly repeated segment of DNA consisting of viral Hin C and Hin A sequences that have recombined with a segment of host DNA that is not highly reiterated in the monkey genome. The monkey segment differs in the two variants as does the size of the viral segment retained. In two additional host-substituted variants, ev-1102 (previously isolated from serial passage 20 by Brockman et al., Virology 54:384-397, 1973) and ev-1108 (newly isolated from serial passage 40), the SV40 sequences derived from the replication origin are present as inverted repetitions. The inverted repeat regions of these two variants have been analyzed at the nucleotide sequence level and are compared with SV40 variant ev-1104 from passage 45 (previously characterized by Gutai and Nathans, J. Mol. Biol. 126:259-274, 1978). The viral segment containing the regulatory signals for replication and viral gene expression is considerably shortened in later serial passages as demonstrated by these five variants. It is of interest that the variants presumably arose due to their enhanced replication efficiency, yet are missing some of the sequence elements implicated in the regulation of replication. Furthermore, a comparison of the structure of the replication origin regions indicates that additional changes occur in the SV40 regulatory region with continued undiluted serial passage.
机译：当猿猴病毒40（SV40）以高多样性连续传代时，会产生自然产生的变体的异质集合。最丰富的那些大概比其他缺陷的和野生型辅助SV40具有选择性复制优势。根据完整的核苷酸序列确定，已经表征了两种这样的天然产生的SV40的宿主取代的变体。进化变种ev-1101（先前由Lee等人，Virology 66：53-69，1975分离）来自未稀释的连续传代13，而ev-2101则新分离自独立衍生的进化系列的未稀释的传代6。两种变体均包含由病毒Hin C和Hin A序列组成的DNA的五次串联重复片段，这些片段已与在猴基因组中没有高度重复的宿主DNA片段重组。猴子节段在两个变体中都不同，所保留的病毒节段的大小也不同。在另外两个宿主取代的变体ev-1102（先前由Brockman等人从串行传代20中分离，病毒学54：384-397，1973）和ev-1108（从串行传代40中新分离）中，SV40序列衍生来自复制起点的序列以反向重复的形式存在。已经在核苷酸序列水平上分析了这两个变体的反向重复区，并将其与来自第45代的SV40变体ev-1104进行了比较（以前由Gutai和Nathans，J. Mol。Biol。126：259-274，1978表征）。 。这五个变体表明，在随后的连续传代中，包含复制和病毒基因表达调控信号的病毒片段会大大缩短。令人感兴趣的是，这些变体大概是由于其增强的复制效率而出现的，但是却缺少一些与复制调控有关的序列元件。此外，复制起点区域结构的比较表明，在连续未稀释的连续传代的情况下，SV40调节区域中发生了其他变化。

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