Control of transcription initiation in vitro requires binding of a transcription factor to the distal promoter of the ovalbumin gene.

M Pastorcic; H Wang; A Elbrecht; S Y Tsai; M J Tsai; B W OMalley

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Control of transcription initiation in vitro requires binding of a transcription factor to the distal promoter of the ovalbumin gene.

机译：体外转录起始的控制需要转录因子与卵清蛋白基因的远端启动子结合。

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We used a cell-free HeLa cell transcription system to identify and characterize transcription factors and the promoter elements that they recognize in RNA polymerase II-transcribed genes. Deletion of the region (-71 to -83) containing the GTCAAA direct repeat resulted in a marked decrease of specific transcription of the ovalbumin gene; transcription could be competed with DNA fragments containing this sequence. Furthermore, DNase I footprinting identified a protein-binding site including this direct repeat with crude extracts and one of the partially purified protein fractions required for transcription. We propose that a soluble factor activates transcription through binding to the direct repeat of GTCAAA sequence upstream from the ovalbumin gene.

机译：我们使用了无细胞的HeLa细胞转录系统来鉴定和表征转录因子及其在RNA聚合酶II转录的基因中识别的启动子元件。删除含有GTCAAA直接重复序列的区域（-71至-83）会导致卵清蛋白基因的特异性转录显着降低;转录可以与含有该序列的DNA片段竞争。此外，DNase I足迹识别了一个蛋白质结合位点，包括与粗提物的直接重复以及转录所需的部分纯化的蛋白质部分。我们建议可溶性因子通过结合卵清蛋白基因上游的GTCAAA序列的直接重复序列来激活转录。

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《Molecular and Cellular Biology》 |1986年第8期|共8页
作者
M Pastorcic; H Wang; A Elbrecht; S Y Tsai; M J Tsai; B W OMalley;
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中图分类细胞生物学;
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1. Control of transcription initiation in vitro requires binding of a transcription factor to the distal promoter of the ovalbumin gene. [J] . M Pastorcic, H Wang, A Elbrecht, Molecular and Cellular Biology . 1986,第8期

机译：体外转录起始的控制需要转录因子与卵清蛋白基因的远端启动子结合。
2. Correct usage of multiple transcription initiation sites and C/EBP-dependent transcription activation of the rat XDH/XO TATA-less promoter requires downstream elements located in the coding region of the gene. [J] . Clark MP, Chow CW, Rinaldo JE, Nucleic Acids Research . 1998,第7期

机译：正确使用大鼠XDH / XO TATA缺失型启动子的多个转录起始位点和C / EBP依赖性转录激活需要位于基因编码区的下游元件。
3. Spot 14 protein interacts and co-operates with chicken ovalbumin upstream promoter-transcription factor 1 in the transcription of the L-type pyruvate kinase gene through a specificity protein 1 (Sp1) binding site. [J] . Compe E, de-Sousa G, Francois K, The Biochemical Journal . 2001,第Pta1期

机译：点14蛋白通过特异性蛋白1（Sp1）结合位点与L型丙酮酸激酶基因转录中的鸡卵清蛋白上游启动子转录因子1相互作用并协同作用。
4. Phylogenetic Analysis of Eubacterial Transcriptional Systems Based On the Dbtbs Database of B. Subtilis Transcription Factors and Promoters [C] . Yuko Makita, naotake Ogasawara, kenta Nakai International Conference on Genome Informatics . 2003

机译：基于B.枯草芽孢杆菌转录因子和启动子DBTBS数据库的有义程学转录系统的系统发育分析
5. The CCAAT-box binding transcription factor, Nuclear Factor-Y (NF-Y) regulates transcription of human aldo-keto reductase 1C1 (AKR1C1) gene. [D] . Pallai, Rajash. 2010

机译：CCAAT盒结合转录因子，核因子-Y（NF-Y）调节人醛糖酮还原酶1C1（AKR1C1）基因的转录。
6. Control of transcription initiation in vitro requires binding of a transcription factor to the distal promoter of the ovalbumin gene. [O] . M Pastorcic, H Wang, A Elbrecht, 1979

机译：体外转录起始的控制需要转录因子与卵清蛋白基因的远端启动子结合。
7. Control of transcription initiation in vitro requires binding of a transcription factor to the distal promoter of the ovalbumin gene. [O] . Pastorcic, M, Wang, H, Elbrecht, A, 1986

机译：体外转录起始的控制需要转录因子与卵清蛋白基因的远端启动子结合。

Control of transcription initiation in vitro requires binding of a transcription factor to the distal promoter of the ovalbumin gene.

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