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首页> 外文期刊>Molecular and Cellular Biology >Characterization of myelin proteolipid mRNAs in normal and jimpy mice.
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Characterization of myelin proteolipid mRNAs in normal and jimpy mice.

机译：正常和j鼠中髓磷脂蛋白脂质mRNA的表征。

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A clone specific for the rat myelin proteolipid protein (PLP) was isolated from a cDNA library made in pUC18 from 17-day-old rat brain stem mRNA. This clone corresponded to the carboxyl-terminal third of the PLP-coding region. The clone was used to identify PLP-specific mRNAs in mouse brain and to establish the time course of PLP mRNA expression during mouse brain development. Three PLP-specific mRNAs were seen, approximately 1,500, 2,400, and 3,200 bases in length, of which the largest was the most abundant. During brain development, the maximal period of PLP mRNA expression was from 14 to 25 days of age, and this was a similar time course to that for myelin basic protein mRNA expression. When the jimpy mouse, an X-linked dysmyelination mutant, was studied for PLP mRNA expression, low levels of PLP mRNA were seen which were approximately 5% of wild-type levels at 20 days of age. When jimpy brain RNA was analyzed by Northern blotting, the PLP-specific mRNA was shown to be 100 to 200 bases shorter than the wild-type PLP-specific mRNA. This size difference was seen in the two major PLP mRNAs, and it did not result from a loss of polyadenylation of these mRNAs.
机译：从17天大的大鼠脑干mRNA的pUC18中制备的cDNA文库中分离出一种对大鼠髓磷脂蛋白脂蛋白（PLP）具有特异性的克隆。该克隆对应于PLP编码区的羧基末端三分之一。该克隆用于鉴定小鼠脑中PLP特异性mRNA，并建立小鼠脑发育过程中PLP mRNA表达的时程。观察到三个PLP特异的mRNA，长度约为1,500、2,400和3,200个碱基，其中最大的是最丰富的。在大脑发育过程中，PLP mRNA表达的最大时期为14至25天，这与髓鞘碱性蛋白mRNA表达的时间过程相似。当研究X连锁的髓鞘异位突变体jimpy小鼠的PLP mRNA表达时，发现低水平的PLP mRNA，大约是20天龄时野生型水平的5％。当通过Northern印迹分析analyzed脑的RNA时，PLP特异性mRNA比野生型PLP特异性mRNA短100至200个碱基。在两个主要的PLP mRNA中发现了这种大小差异，但这不是由于这些mRNA的聚腺苷酸化损失所致。

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