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首页> 外文期刊>Molecular and Cellular Biology >A calcium ionophore-inducible cellular promoter is highly active and has enhancerlike properties.
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A calcium ionophore-inducible cellular promoter is highly active and has enhancerlike properties.

机译：钙离子载体诱导的细胞启动子是高活性的，并具有增强子样特性。

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We examined the regulatory/promoter sequence of a calcium ionophore-inducible gene isolated from the rat genome. Whereas the promoter of this ubiquitously expressed gene is active under noninduced conditions, after induction by calcium ionophore A23187 this promoter is 10- to 25-fold more active than the simian virus 40 early promoter, as measured by chloramphenicol acetyltransferase activities. Within this regulatory/promoter region, we have identified a DNA fragment with enhancer-like properties immediately 5' to the TATA sequence. This 291-nucleotide fragment acts in cis to enhance expression of the neomycin phosphotransferase (neo) gene driven by the herpes simplex virus thymidine kinase promoter in an orientation-independent manner. In addition, this fragment can confer A23187 inducibility to the neo gene and effectively compete for positive regulatory factors involved in A23187 induction. Sequence analysis of this promoter reveals homology with viral core enhancer sequences, and the apparent organization of direct repeat domains is similar to those observed in viral enhancers.
机译：我们检查了从大鼠基因组中分离出的钙离子载体可诱导基因的调控/启动子序列。尽管该普遍表达的基因的启动子在非诱导条件下是有活性的，但在钙离子载体A23187诱导后，该启动子的活性比猿猴病毒40早期启动子的活性高10至25倍（如氯霉素乙酰转移酶活性所测定）。在这个调控/启动子区域内，我们已经鉴定出一个与TATA序列紧靠5'的具有增强子样特性的DNA片段。该291个核苷酸片段顺式作用，以定向独立的方式增强由单纯疱疹病毒胸苷激酶启动子驱动的新霉素磷酸转移酶（neo）基因的表达。另外，该片段可以赋予A23187对neo基因的诱导性，并有效竞争涉及A23187诱导的阳性调节因子。该启动子的序列分析揭示了与病毒核心增强子序列的同源性，并且直接重复结构域的表观组织与在病毒增强子中观察到的相似。

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