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首页> 外文期刊>Molecular and Cellular Biology >c-mos proto-oncogene RNA transcripts in mouse tissues: structural features, developmental regulation, and localization in specific cell types.
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c-mos proto-oncogene RNA transcripts in mouse tissues: structural features, developmental regulation, and localization in specific cell types.

机译：小鼠组织中的c-mos原癌基因RNA转录物：结构特征，发育调控和特定细胞类型的定位。

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c-mos RNA transcripts have been previously detected in mouse gonadal tissue and in late-term embryos. Here, we show that they are also present at low levels in placenta and in adult mouse brain, kidney, mammary gland, and epididymis. Marked differences are observed in the size of the mos RNA transcripts detected in different tissues. All transcripts appear to end at the same 3' position, and the tissue-specific size variations appear to be due to the use of different promoters. For example, the testicular and ovarian RNA transcripts initiate approximately 280 and approximately 70 base pairs, respectively, upstream from the first initiation codon, but both end at a common site downstream from the mos open reading frame. The expression of mos is developmentally regulated in gonadal tissue. Thus, the level of mos transcripts in testes is low for the first 3 weeks after birth, increases at least 10-fold around day 25, and reaches adult levels by day 30. In contrast, ovaries from preweaning mice contain a higher level of mos mRNA compared to ovaries from adult mice. In cell fractionation experiments we show that mos transcripts are present in haploid germ cells. We find that these transcripts are associated with monosomes and polysomes. The peculiar pattern of mos expression in mouse gonadal tissue suggests a role for the c-mos proto-oncogene in germ cell differentiation.
机译：先前已在小鼠性腺组织和晚期胚胎中检测到c-mos RNA转录本。在这里，我们显示它们在胎盘中以及成年小鼠的大脑，肾脏，乳腺和附睾中也以低水平存在。在不同组织中检测到的mos RNA转录物的大小存在明显差异。所有的转录本似乎都在相同的3'位置终止，并且组织特异性大小的变异似乎是由于使用了不同的启动子。例如，睾丸和卵巢RNA转录物分别在第一个起始密码子的上游起始约280个碱基对和约70个碱基对，但均在mos开放阅读框下游的一个公共位点终止。 mos的表达在性腺组织中受到发育调节。因此，出生后前三周睾丸中的mos转录水平很低，在第25天左右增加至少10倍，到第30天达到成年水平。相比之下，断奶前小鼠的卵巢中mos的水平更高mRNA与成年小鼠卵巢相比。在细胞分离实验中，我们显示mos转录本存在于单倍体生殖细胞中。我们发现这些成绩单与单核糖体和多核糖体相关。小鼠性腺组织中mos表达的特殊模式表明c-mos原癌基因在生殖细胞分化中的作用。

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