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首页> 外文期刊>Molecular and Cellular Biology >Mapping functional regions of transcription factor TFIIIA.
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Mapping functional regions of transcription factor TFIIIA.

机译：映射转录因子TFIIIA的功能区。

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Functional deletion mutants of the trans-acting factor TFIIIA, truncated at both ends of the molecule, have been expressed by in vitro transcription of a cDNA clone and subsequent cell-free translation of the synthetic mRNAs. A region of TFIIIA 19 amino acids or less, near the carboxyl terminus, is critical for maximal transcription and lies outside the DNA-binding domain. The elongated protein can be aligned over the internal control region (ICR) of the Xenopus 5S RNA gene with its carboxyl terminus oriented toward the 5' end of the gene and its amino terminus oriented toward the 3' end of the gene. The nine "zinc fingers" and the linkers that separate them comprise 80% of the protein mass and correspond to the DNA-binding domain of TFIIIA. The zinc fingers near the amino terminus of the protein contribute more to the overall binding energy of the protein to the ICR than do the zinc fingers near the carboxyl end. The most striking feature of TFIIIA is its modular structure. This is demonstrated by the fact that each zinc finger binds to just one of three short nucleotide sequences within the ICR.
机译：反式作用因子TFIIIA的功能性缺失突变体在分子的两端被截断，已通过cDNA克隆的体外转录和随后合成mRNA的无细胞翻译来表达。靠近羧基末端的TFIIIA 19个氨基酸或更少的区域对于最大转录至关重要，并且位于DNA结合域之外。伸长的蛋白可以在非洲爪蟾5S RNA基因的内部控制区域（ICR）上对齐，其羧基末端朝向基因5'端，氨基末端朝向基因3'端。九个“锌指”和分隔它们的接头占蛋白质质量的80％，对应于TFIIIA的DNA结合结构域。靠近蛋白质氨基末端的锌指比靠近羧基末端的锌指对蛋白质与ICR的整体结合能的贡献更大。 TFIIIA的最大特点是其模块化结构。每个锌指仅与ICR中三个短核苷酸序列之一结合的事实证明了这一点。

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