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首页> 外文期刊>Molecular and Cellular Biology >Suppression of chromosomal mutations affecting M1 virus replication in Saccharomyces cerevisiae by a variant of a viral RNA segment (L-A) that encodes coat protein.
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Suppression of chromosomal mutations affecting M1 virus replication in Saccharomyces cerevisiae by a variant of a viral RNA segment (L-A) that encodes coat protein.

机译：通过编码外壳蛋白的病毒RNA片段（L-A）的变体抑制影响酿酒酵母M1病毒复制的染色体突变。

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For the maintenance of "killer" M1 double-stranded RNA in Saccharomyces cerevisiae, more than 30 chromosomal genes are required. The requirement for some of these genes can be completely suppressed by a cytoplasmic element, [B] (for bypass). We have isolated a mutant unable to maintain [B] (mab) and found that it is allelic to MAK10, one of the three chromosomal MAK genes required for the maintenance of L-A. The heat curing of [B] always coincided with the loss of L-A. To confirm that [B] is located on L-A, we purified viral particles containing either L-A or M1 from strains with or without [B] activity and transfected these purified particles into a strain which did not have either L-A or M1. The transfectants harboring L-A and M1 from a [B] strain showed the [B] phenotype, but the transfectants with L-A and M1 from a [B-o] strain did not show the [B] phenotype. Furthermore, the transfectants having L-A from a [B] strain and M1 from a [B-o] strain also showed the [B] phenotype. Therefore, we concluded that [B] is a property of a variant of L-A. In the transfection experiment, we also proved that the superkiller phenotype of the [B] strain is a property of L-A and that L-A with [B] activity can maintain a higher copy number of M1 regardless of the source of M1 viruslike particles. These data suggest that MAK genes whose mutations are suppressed by [B] are concerned with the protection of M1 (+) single-stranded RNA or the formation of M1 viruslike particles and that an L-A with more efficient production of M1 viruslike particles can completely dispense with the requirement for those MAK genes.
机译：为了在酿酒酵母中维持“杀手” M1双链RNA，需要30多个染色体基因。这些基因中某些基因的需求可以完全被细胞质元素[B]抑制（用于旁路）。我们分离出一个无法维持[B]（mab）的突变体，发现该突变体与MAK10等位基因，MAK10是维持L-A所需的三个染色体MAK基因之一。 [B]的热固化总是与L-A的损失同时发生。为了确认[B]位于L-A上，我们从具有[B]活性或没有[B]活性的菌株中纯化了含有L-A或M1的病毒颗粒，然后将这些纯化的颗粒转染到既没有L-A又没有M1的菌株中。带有[B]菌株的L-A和M1的转染子显示[B]表型，但是带有[B-o]菌株的L-A和M1的转染子没有显示[B]表型。此外，具有来自[B]菌株的L-A和来自[B-o]菌株的M1的转染子也显示[B]表型。因此，我们得出结论，[B]是L-A变体的属性。在转染实验中，我们还证明了[B]菌株的超级杀手表型是L-A的特性，具有[B]活性的L-A可以保持更高的M1拷贝数，而与M1病毒样颗粒的来源无关。这些数据表明，其突变被[B]抑制的MAK基因与M1（+）单链RNA的保护或M1病毒样颗粒的形成有关，而更高效生产M1病毒样颗粒的LA可以完全分配对那些MAK基因的需求。

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