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首页> 外文期刊>Molecular and Cellular Biology >Transcriptional and post-transcriptional regulation of globin gene accumulation in murine erythroleukemia cells.
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Transcriptional and post-transcriptional regulation of globin gene accumulation in murine erythroleukemia cells.

机译：小鼠红白血病细胞中球蛋白基因积累的转录和转录后调控。

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摘要

The mechanism responsible for the accumulation of newly synthesized alpha- and beta-globin mRNA in the cytoplasm of induced murine erythroleukemia cells was examined by nuclear mRNA nascent chain elongation (run-off transcription). Hexamethylenebisacetimide, a potent inducer of murine erythroleukemia cell differention, induced high levels of both alpha- and beta-globin gene transcription within 48 to 72 h in culture. Butyric acid, a modest inducer of murine erythroleukemia cells, induced a somewhat lower level of globin gene transcription. With both inducers, alpha-globin transcriptional rates exceeded those of beta-globin. Hemin, on the other hand, showed no detectable increase over the basal rate observed in uninduced cells, even at a time (48 h) when newly synthesized globin mRNA was accumulating in the cytoplasm. These results suggest that there are at least two mechanisms responsible for regulating alpha- and beta-globin structural gene expression in induced murine erythroleukemia cells and that the mechanisms involved are inducer dependent. Hexamethylenebisacetimide and butyric acid increase the rate at which globin genes are transcribed, but hemin appears to allow constitutive levels of transcripts to accumulate.
机译：通过核mRNA新生链延长（径流转录）检查了导致新合成的α-和β-珠蛋白mRNA在诱导的鼠红白血病细胞的细胞质中积累的机制。六亚甲基双乙酰亚胺（一种有效的鼠类红细胞白血病细胞分化诱导剂）在培养的48至72小时内诱导了高水平的α-和β-珠蛋白基因转录。丁酸是鼠类红细胞白血病细胞的温和诱导剂，其诱导的球蛋白基因转录水平较低。在两种诱导剂下，α-珠蛋白的转录速率都超过了β-珠蛋白的转录速率。另一方面，即使在新合成的球蛋白mRNA在细胞质中蓄积的时间（48小时），血红素也未显示出在未诱导细胞中观察到的基础速率上没有可检测到的增加。这些结果表明，至少有两种机制可调节诱导的鼠红白血病细胞中的α-和β-球蛋白结构基因表达，并且所涉及的机制是诱导物依赖性的。六亚甲基双乙酰亚胺和丁酸增加了珠蛋白基因的转录速度，但血红素似乎可以使转录本的组成水平得以积累。

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